We narrowed to 4,121 results for: LYC;

1. pHIV-EGFP.Flag-EZH2-mt2*

   Plasmid

   #220246

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2* (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-FKO (C-flag)

   Plasmid

   #159364

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExp-RBD-CHis

   Plasmid

   #195002

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Tags

   His8

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PF3D7_1136200-bio

   Plasmid

   #47730

   Purpose

   Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised PF3D7_1136200
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin replaced…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-SARS2-S-FKO (NC-flag)

   Plasmid

   #159363

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag and Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-SARS2-S-G614 (NC-flag)

   Plasmid

   #156419

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3322_pETcon_SARS2_Omicron-BA2

   Plasmid

   #184409

   Purpose

   yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

   Plasmid

   #160568

   Purpose

   tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_1-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

   Plasmid

   #169469

   Purpose

   Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECE-M2-AMPKalpha2 M93G

    Plasmid

    #31653

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid

    #75411

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [M1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE-HA-AMPKalpha2 M93G

    Plasmid

    #31655

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. EBL1-bio

    Plasmid

    #47792

    Purpose

    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBL1
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

    Plasmid

    #166019

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti PGK Puro GFP-GABARAPL1 G116A

    Plasmid

    #123245

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    PGK

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

    Plasmid

    #169467

    Purpose

    Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-LN

    Plasmid

    #188231

    Purpose

    Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Insert

    LIN28A and NANOG separated by 2A sequence (LIN28A, NANOG Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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