We narrowed to 6,017 results for: cki;

1. pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

   Plasmid

   #207508

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HLA-cMyc-EcopT1R1

   Plasmid

   #113962

   Purpose

   mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R1 (TAS1R1 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   truncate N-terminal 24 residues

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-Flag-KIAA0947

   Plasmid

   #49248

   Purpose

   Mammalian expression of flag-tagged human KIAA0947

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   KIAA0947 (ICE1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMT-Hrp48-ADARcd-V5

   Plasmid

   #81172

   Purpose

   identification of cell-specific targets of RNA binding proteins

   Depositor

   Michael Rosbash

   Article

   McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

   Insert

   Hrp48-ADARcd fusion (Hrb27C Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila metallothionein promoter

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. INS-2A-luciferase-2A-CD19-GFP

   Plasmid

   #159349

   Purpose

   Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Luciferase-2A-human_CD19-2A-GFP
   Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
   

   Use

   Ins locus knockin donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC22A31

   Plasmid

   #132036

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A31 (SLC22A31 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC2A4

   Plasmid

   #132102

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A4 (SLC2A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221_SLC6A14

   Plasmid

   #131865

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A14 (SLC6A14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRK5-myc-Miro2 E208K/E328K

    Plasmid

    #47900

    Purpose

    Expresses myc tagged Miro2 V208K/E328K mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 E208K/E328K (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    E208K/E328K, abolishes calcium binding

    Promoter

    CMV

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pJW2086

    Plasmid

    #154335

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-TAPBPR-TM

    Plasmid

    #178645

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC35A3

    Plasmid

    #132135

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35A3 (SLC35A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221_SLC5A2

    Plasmid

    #132241

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A2 (SLC5A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. hHS1-M7A,H102A

    Plasmid

    #159171

    Purpose

    Human codon-optimized cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    hHS1-M7A,H102A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Sac-Aβ(M1-42/C18C33)

    Plasmid

    #173759

    Purpose

    Expresses the AβC18C33 peptide.

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

    Insert

    Aβ(M1-42/C18C33) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed valine 18 to cysteine; changed glycine 33…

    Promoter

    T7

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACE2-Fc

    Plasmid

    #164222

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 extracellular domain (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-Flag-Rheb-4Rs

    Plasmid

    #165022

    Purpose

    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-4Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    change lysine residues on position 109, 135, 151,…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid

    #113957

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG, Signal/leader sequence from influenza hemag…

    Expression

    Mammalian

    Mutation

    residues 22-568 fused to a transmembrane tether

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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