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pGBW-m4046914

Plasmid

#145738

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp9

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046839

Plasmid

#145646

Purpose

Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046813

Plasmid

#145619

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046770

Plasmid

#145594

Purpose

Bacterial Expression plasmid for SARS-CoV-2 leader protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046780

Plasmid

#145736

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp10

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046964

Plasmid

#145681

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp3

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4095419

Plasmid

#149526

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp3

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

d1-1088 d1203-1945

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046950

Plasmid

#149258

Purpose

Bacterial expression plasmid for SARS-CoV-2 ORF10 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046856

Plasmid

#145698

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNH078

Plasmid

#42164

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046831

Plasmid

#145671

Purpose

Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;Myc

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDS66

Plasmid

#48364

Purpose

3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNI2

Plasmid

#30997

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NI2

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMST665_BB3_L 23_gRNAempty_cas9_BbsI

Plasmid

#90278

Purpose

BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

Cas9

Use

A. niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHD4

Plasmid

#30977

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD4

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pDS67

Plasmid

#48365

Purpose

3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046906

Plasmid

#149248

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046797

Plasmid

#145676

Purpose

Bacterial Expression plasmid for SARS-CoV-2 leader protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHD7

Plasmid

#30980

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD7

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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