We narrowed to 4,310 results for: p2a

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RPPH1 (pAVA3585)

Plasmid

#239262

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-CCRB

Plasmid

#236772

Purpose

A piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

TRE3G promoter, CAG promoter

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.1-TEV

Plasmid

#234518

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV:: NES-FKBP-iLID-LRP6c(△1-64)

Plasmid

#221775

Purpose

Expresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: NES-FKBP-iLID-LRP6c

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP +0

Plasmid

#194308

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -2

Plasmid

#194313

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -0

Plasmid

#194311

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -1

Plasmid

#194312

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

Plasmid

#161534

Purpose

Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF511

Plasmid

#104037

Purpose

Donor vector for 3' FLAG tag of human ZNF511

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 homology arms (ZNF511 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_RFXANK

Plasmid

#86296

Purpose

Donor vector for 3' FLAG tag of human RFXANK

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RFXANK homology arms (RFXANK Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228555

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA and P2A-mCherry

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-2A-Cas9

Plasmid

#170805

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

Depositor

Morten Frodin

Article

Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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