161,805 results

1. pMYT013

   Plasmid

   #180666

   Purpose

   Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

   Depositor

   Tom Ellis

   Article

   Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

   Insert

   pTet5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

   Plasmid

   #163727

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding three amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.3TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,N135TAG,Y151TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSB-hsa-miR-151a-3p

   Plasmid

   #103258

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-151a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-151a-3p target (MIR151A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-151a-5p

   Plasmid

   #103259

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-151a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-151a-5p target (MIR151A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-let-7b-3p

   Plasmid

   #103147

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-let-7b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-let-7b-3p target (MIRLET7B Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_antiCD19_synNotch_Gal4VP64

   Plasmid

   #79125

   Purpose

   Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   antiCD19_synNotch_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-UGAcS

   Plasmid

   #172135

   Purpose

   mammalian expression plasmid for a UDP-GlcNAc sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UGAcS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-Tight-MitoTimer

    Plasmid

    #50547

    Purpose

    Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

    Depositor

    Roberta Gottlieb

    Article

    Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

    Insert

    MitoTimer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F12S mutation in Timer

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. HyPer7.2DAAO-mito

    Plasmid

    #168304

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-mito
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Promoter

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPROBE-NT

    Plasmid

    #37818

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-I-SceI

    Plasmid

    #60960

    Purpose

    Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    I-SceI restriction enzyme
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 HaloTag-V5-TurboID

    Plasmid

    #226474

    Purpose

    Expresses fusion of HaloTag and TurboID in mammalian cells

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

    Insert

    TurboID
    

    Use

    Tags

    HaloTag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge