164,577 results

1. pAAV-TRE-NpHR-EYFP

   Plasmid

   #171621

   Purpose

   TRE-NpHR-EYFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-NpHR-EYFP
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Mm.cargo(Cre)

   Plasmid

   #174862

   Purpose

   Expresses mouse Peg10 cargoRNA encoding Cre recombinase

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   Cre flanked by MmPeg10 UTRs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-ESR1-Y537S

   Plasmid

   #116374

   Purpose

   Lentiviral expression of ESR1 Y537S

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y537S

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAF020 p3E-2A-mCerulean

   Plasmid

   #195974

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple Ga11

   Plasmid

   #196059

   Purpose

   Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha11-RLuc8 (GNA11 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 4xCSL-luciferase

   Plasmid

   #41726

   Depositor

   Raphael Kopan

   Article

   Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

   Insert

   4xCSL binding sites
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains a multimerized high affinity CSL binding…

   Promoter

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBMN BFP-Parkin

   Plasmid

   #186221

   Purpose

   For expression of Parkin tagged with BFP

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ150

   Plasmid

   #69301

   Purpose

   Gateway entry vector with pcoCas9 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9 (plant codon-optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR2.1 Clover-LMNA Donor

    Plasmid

    #122508

    Purpose

    Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
    

    Use

    Homology repair template plasmid (donor plasmid)

    Tags

    Clover

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRUPATH Triple Gai2

    Plasmid

    #196049

    Purpose

    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-6xGLY-3xFLAG-hphMX4

    Plasmid

    #20755

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TDP43-NTD

    Plasmid

    #241024

    Purpose

    Bacterial expression of TDP-43 NTD

    Depositor

    Nicolas Fawzi

    Insert

    TDP43-NTD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    changed N81 to stop codon

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid

    #189743

    Purpose

    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A2 (ALDH1A2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid

    #229498

    Purpose

    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC 5'Untranslated region + destabilized GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge