We narrowed to 4,207 results for: biorxiv

1. pCAG_ATG101

   Plasmid

   #223796

   Purpose

   Expression of recombinant protein for purification (co-expression with ATG13)

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   ATG101 (ATG101 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG_GST-TEV-WIPI1

   Plasmid

   #223798

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI1 (WIPI1 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGADT7_Sr62NLRa

   Plasmid

   #233528

   Purpose

   Expresses Sr62NLRa in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62NLRa
   

   Use

   Tags

   GAL4 AD, HA tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hnRNPA1_D262V-FLAG-IRES-eGFP

   Plasmid

   #234620

   Purpose

   Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1 D262V (HNRNPA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Famillial ALS mutation D262V, C-terminal FLAG tag…

   Promoter

   CMV

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hnRNPA1-FLAG-IRES-eGFP

   Plasmid

   #234619

   Purpose

   Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C-terminal FLAG tag, co-expressing with eGFP via …

   Promoter

   CMV

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. A1-LCD 5W D262V

   Plasmid

   #234617

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_5W D262V (HNRNPA1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   F222W, F228W, Y237W, D262V, Y305W, F320W

   Promoter

   T7

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

   Plasmid

   #229807

   Purpose

   A CAGGS driven tet-on transactivator with an nls-EGFP reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   rtTA-IRES-nls-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

   Plasmid

   #229811

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA transactivator-LexA-Operon-nls-GFP
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

   Plasmid

   #229812

   Purpose

   A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   nls-mCherry
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

    Plasmid

    #229813

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    LexA-mODC-LexAOp-cFos
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

    Plasmid

    #229820

    Purpose

    An AAV, tet-off, gateway compatible destination vector with cre dependent expression of the insert. EFS driving destablized rTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

    Plasmid

    #229823

    Purpose

    A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

    Plasmid

    #229838

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    LexA-mODC-LexAOp
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling follwed by and IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Lamin nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge