We narrowed to 18,356 results for: Tec

1. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-TetR-Rad18UBD

   Plasmid

   #119010

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCKan-yibDp-GST

   Plasmid

   #134592

   Purpose

   Low Phosphate inducible GST

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   Glutathione S-transferase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE252

   Plasmid

   #120791

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GGE253

   Plasmid

   #120790

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp211-P30-His-POL30-3'

   Plasmid

   #99546

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLIP43

   Plasmid

   #65618

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edAFPt9-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19/mlcR25-mEGFP

    Plasmid

    #154295

    Purpose

    Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB4132

    Plasmid

    #106127

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET FLIPE-1m

    Plasmid

    #13540

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P shMOCS3_2

    Plasmid

    #102974

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_2 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P shMOCS3_1

    Plasmid

    #102973

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_1 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBT362

    Plasmid

    #127844

    Purpose

    clone BE4max-NG CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max-NG without deaminase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST1374-Lb-Cpf1-NLS

    Plasmid

    #107680

    Purpose

    Expresses LbCpf1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    LbCpf1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6Rosa-CAG-Cas9

    Plasmid

    #118998

    Purpose

    Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 S100A1

    Plasmid

    #67048

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    S100A1 (S100A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only