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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

Plasmid

#72639

Purpose

Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain

Depositor

Joshua Bonkowsky

Insert

UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)

Use

Gateway

Mutation

Stop codon after mCherry- SEE DEPOSITOR COMMENTS …

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag (S89A)

Plasmid

#19026

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, S89A (site directed mu…

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-Slug

Plasmid

#36986

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

slug (Snai2 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Cntn1-Fc-His

Plasmid

#72065

Purpose

Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn1 (Cntn1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-Lyl1

Plasmid

#131612

Purpose

expression of mouse LyI1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Lyl1 (Lyl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]

Plasmid

#16283

Depositor

Thomas Jessell

Article

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Insert

HB9 promoter (Mnx1 Mouse)

Use

Mouse Targeting

Tags

IRES-EGFP

Expression

Mammalian

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pDendra2-H4

Plasmid

#157815

Purpose

Fluorescent reporter for histone H4 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H4 (H4c14 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Palladin-C-7

Plasmid

#55113

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Palladin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pSK-mPar1

Plasmid

#53227

Purpose

bacterial expression of mouse Par1

Depositor

Shaun Coughlin

Insert

Par1 (F2r Mouse)

Expression

Bacterial

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only
pIS1-Actb5UTR-renilla

Plasmid

#38234

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Actb 5'UTR / Renilla (Actb Mouse)

Expression

Mammalian

Mutation

Actb 5' UTR fused to renilla ORF

Promoter

Endogenous Actb

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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FoxO1 KQ

Plasmid

#66165

Purpose

Expresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.

Depositor

Ronald Kahn

Article

Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

Insert

FoxO1 KQ (Foxo1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

K242Q, K245Q and K262Q

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only
pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
MyD116.PFLAG.CMV2

Plasmid

#21834

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 (Ppp1r15a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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hsp70:Arl13b-mKate2

Plasmid

#105964

Purpose

heat shock inducible transgenesis, primary cillium marker

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Arl13b (Arl13b Mouse)

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH

Plasmid

#105981

Purpose

This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

Depositor

Marcel Bruchez

Article

Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

Insert

hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH

Use

AAV

Tags

FAPdL5-POST, T2A-dTomato, and myc

Promoter

human synapsin 1

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_Sik3

Plasmid

#119972

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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