We narrowed to 22,821 results for: Fus

1. pCB6-HAOccludinCT3

   Plasmid

   #136640

   Purpose

   Expression of HAOccludinCT3 in mammalian cells

   Depositor

   Karl Matter, Maria Balda

   Article

   Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

   Insert

   HAOccludinCT3 (OCLN Chicken)
   

   Use

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal cytoplasid domain

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME-mKate2-GPI-pA

   Plasmid

   #109542

   Purpose

   Multisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pProEx-Htb-cytATL2-6Gly-sfGFP

   Plasmid

   #124065

   Purpose

   E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   cytATL-sfGFP
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBDU-Atg17(F317D, I325A)

   Plasmid

   #122275

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid; Isoleucine 32…

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGAD-Atg17(F317H)

   Plasmid

   #122269

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Histidine

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBDU-Atg17(I325A)

   Plasmid

   #122274

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Isoleucine 325 to alanine

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGAD-Atg17(F317D, I325A)

   Plasmid

   #122273

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid; Isoleucine 32…

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGAD-Atg17(I325A)

   Plasmid

   #122272

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Isoleucine 325 to alanine

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAD-Atg17(F317Q)

   Plasmid

   #122271

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to glutamine

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAD-Atg17(F317D)

    Plasmid

    #121391

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGAD-Atg17(F317G)

    Plasmid

    #122268

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to glycine

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGAD-Atg17(F317K)

    Plasmid

    #122270

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to lysine

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAD-Atg17(∆335-417)

    Plasmid

    #121525

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 335-417*

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBDU-Atg17(L105D)

    Plasmid

    #121395

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 105 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBDU-Atg17(I325D)

    Plasmid

    #121397

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAD-Atg17(L331D)

    Plasmid

    #121393

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 331 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAD-Atg17(L105D)

    Plasmid

    #121388

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 105 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGAD-Atg17(I325D)

    Plasmid

    #121392

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAD-Atg17(I310D)

    Plasmid

    #121389

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 310 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBDU-Atg17(F317D)

    Plasmid

    #121396

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid

    Promoter

    ADH1 promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGAD-Atg17(I98E)

    Plasmid

    #121387

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 98 to Glutamic acid

    Promoter

    ADH1 promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGAD-Atg17(∆92-106)

    Plasmid

    #121517

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 92-106

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGAD-Atg17(∆313-334)

    Plasmid

    #121524

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 313-334

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGAD-Atg17(∆296-312)

    Plasmid

    #121523

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 296-312

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGAD-Atg17(∆279-295)

    Plasmid

    #121522

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 279-295

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAD-Atg17(∆129-177)

    Plasmid

    #121518

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 129-177

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGAD-Atg17(∆266-280)

    Plasmid

    #121520

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 266-280

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGAD-Atg17(∆15-106)

    Plasmid

    #121515

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 15-106

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAD-Atg17(∆186-236)

    Plasmid

    #121519

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 186-236

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGAD-Atg17(∆266-312)

    Plasmid

    #121521

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 266-312

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b+2(L171C)X

    Plasmid

    #108192

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC3R112C/T180C

    Plasmid

    #85088

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3

    Promoter

    T7 promoter

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCALNL_Ch5_155183064-155183141

    Plasmid

    #81211

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTCW018

    Plasmid

    #83531

    Purpose

    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pARO9/pFUS1J2

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLKO1-puro-U6-sgRNA-PLXNB2

    Plasmid

    #69240

    Purpose

    U6 driven SpCas9 sgRNA expression for PLXNB2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    PLXNB2 sgRNA (PLXNB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

    Plasmid

    #69221

    Purpose

    Expresses R1333K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

    Plasmid

    #69222

    Purpose

    Expresses R1333S mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Serine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMSCV-puro-mMCL1 Delta C22

    Plasmid

    #45820

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    mMCL-1 Delta C22
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal 22 amino acids are deleted from full l…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

47. pMSCV-puro-Delta N67-mMCL1

    Plasmid

    #45819

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    Delta N67-mMCL-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-terminus 67 amino acids deleted from full lengt…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pFSW Syt1-C2B V304A,I367A IRES GFP

    Plasmid

    #133821

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    V304A, I367A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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49. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pFSW Syt1-C2A K189-192A IRES GFP

    Plasmid

    #133823

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    K189-192A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge