We narrowed to 42,145 results for: Eras

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pCR 2.1-TOPO Fzd6 S354I HA

Plasmid

#208364

Purpose

Extracellular HA-tagged Fzd6 S354I (mouse)

Depositor

Maureen Cetera, Danelle Devenport

Article

Cetera et al Development. 2023 Aug 25:dev.202078. doi

Insert

Fzd6 (Fzd6 Mouse)

Use

Cloning vector

Tags

HA

Mutation

Serine 354 to Isoleucine

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCR 2.1-TOPO Fzd6HA

Plasmid

#208363

Purpose

Extracellular HA-tagged Fzd6 (mouse)

Depositor

Maureen Cetera, Danelle Devenport

Article

Cetera et al Development. 2023 Aug 25:dev.202078. doi

Insert

Fzd6 (Fzd6 Mouse)

Use

Cloning vector

Tags

HA

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-LS

Plasmid

#205438

Purpose

Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

Depositor

Masamichi Kamihira

Article

Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

Inserts

Gaussia luciferase gene
spectinomycin resistance gene

Use

Cre/Lox and Luciferase; Chlamydomonas expression

Promoter

Heat shock protein 70A (HSP70A)/ribulose bisphosp…

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRANS-Pho2-1/2-2-Csy4

Plasmid

#161763

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Expression

Plant

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTRANS-Pho2-1/2-2-tRNA

Plasmid

#161762

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Expression

Plant

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

Plasmid

#197959

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

2x35S promoter and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

Plasmid

#197960

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197980

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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hIgG1-Ty1-hFc

Plasmid

#194593

Purpose

Mammalian expression of Ty1 nanobody upstream of human IgG1

Depositor

Gerald McInerney

Article

Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

Insert

Ty1-hFc (S )

Expression

Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-SRO1 JN177

Plasmid

#192546

Purpose

pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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CHI +N122D

Plasmid

#187427

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed asparagine at 130 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Cyp26a1

Plasmid

#185563

Purpose

AAV construct to ubiquitously express mouse Cyp26a1

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

Cyp26a1 (Cyp26a1 Mouse)

Use

AAV

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_15278a

Plasmid

#173545

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_15278_P17777

Expression

Plant

Promoter

35S

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Ovol1

Plasmid

#172871

Purpose

Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Ovol1 (Ovol1 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_14371

Plasmid

#173567

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_14371_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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