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Showing: 1681 - 1690 of 170787 results

  2. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189755-plasmid-map-sequence-id-385774

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  6. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  7. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92120-plasmid-map-sequence-id-197614

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  8. SpRY-ABE8e(V106W)

    Plasmid
    #198553
    Purpose
    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198553-plasmid-map-sequence-id-388905

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