2,223 results
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Plasmid#11302DepositorInsertIgZ (ighz Zebrafish)
ExpressionBacterialAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)
Plasmid#242593PurposeA Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.DepositorInsertmCerulean-zGeminin, mCherry-zCdt1
UseZebrafish expressioPromoterzebrafish UbiAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…Promotermyo6bAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk3
Plasmid#207614PurposeZebrafish Alk3 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-BMPR2a
Plasmid#207616PurposeZebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOVDepositorInsertBMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Zebrafish, Vaucheria frigida)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:redCas9; control_gRNA
Plasmid#199334PurposepDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNADepositorInsertszCREST3
Cas9
control sgRNA
UseTol2 destination vectorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -0
Plasmid#180010PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin4b-Clover
Plasmid#163854PurposeZebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZDepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin1a-Clover
Plasmid#163852PurposeZebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPRDepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-H2B-pr-mEosFP
Plasmid#141101PurposeH2B fused to pr-mEosFP for chromatin visualization and primed conversionDepositorInsertHistone 2B
Tagspr-mEosFPExpressionMammalianPromotersimian CMV IE94Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Trpa1b-FLAG
Plasmid#106403PurposeMammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.DepositorAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
Plasmid#140893PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian celDepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE), cha…Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME DN-E-cadherin
Plasmid#135209PurposeDominate Negative zebrafish e-cadherin labelDepositorInsertCDH1
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-Kaede
Plasmid#132976Purposegata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backboneDepositorInsertsKaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECEbasp
Plasmid#132968Purposegata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6) with a b-actin basal promoter
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only