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We narrowed to 504 results for: ubiquitin plasmid

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Showing: 481 - 504 of 504 results

  5. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  6. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  7. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  8. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  9. pAAV-UBC-S2-cAMPr

    Plasmid
    #160723
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-cAMPr
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160723-plasmid-map-sequence-id-315524

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  10. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  11. pAAV-UBC-S3-ExRaiAKAR

    Plasmid
    #160724
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-ExRaiAKAR
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160724-plasmid-map-sequence-id-315754

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  12. pAAV-UBC-S4-ExRaiCKAR

    Plasmid
    #160725
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S4-ExRaiCKAR
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160725-plasmid-map-sequence-id-315528

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  13. pAAV-UBC-S2-RAB_EKARev

    Plasmid
    #160726
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-RAB_EKARev
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160726-plasmid-map-sequence-id-315763

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  15. pAAV-UBC-S3-RAB_EKARev

    Plasmid
    #160727
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-RAB_EKARev
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160727-plasmid-map-sequence-id-315764

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  16. pAAV-UBC-S1-jRCaMP1a

    Plasmid
    #160728
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-jRCaMP1a
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160728-plasmid-map-sequence-id-315534

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  18. Lenti-LucAL

    Plasmid
    #174049
    Purpose
    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucAL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…
    Promoter
    Human Ubiquitin C
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    174049-plasmid-map-sequence-id-345204

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  19. Lenti-LucA

    Plasmid
    #174047
    Purpose
    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucA
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine
    Promoter
    Human Ubiquitin C
    Availability
    Academic Institutions and Nonprofits only
    174047-plasmid-map-sequence-id-345203

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  20. Lenti-LucL

    Plasmid
    #174048
    Purpose
    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    LAMA4 glycine 1254 to valine
    Promoter
    Human Ubiquitin C
    Availability
    Academic Institutions and Nonprofits only
    174048-plasmid-map-sequence-id-345139

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  24. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Promoter
    Aedes aegypti Polyubiquitin
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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Showing: 481 - 504 of 504 results