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Showing: 801 - 850 of 167150 results
  1. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Insert
    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 850 of 167150 results