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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,220 results for: sumo

Showing: 801 - 850 of 1220 results

  1. 5UBB (LOC149281)

    Plasmid
    #101257
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL11B (METTL11B Human)
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101257-plasmid-map-sequence-id-197640

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  2. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Promoter
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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  4. 6DUB (LOC149281)

    Plasmid
    #119967
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    LOC149281 (METTL11B Human)
    Use
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 58-278
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119967-plasmid-map-sequence-id-232691

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  19. polySIM

    Plasmid
    #221068
    Purpose
    Expression of polySIM protein under T7 promoter
    Depositor
    Michael Rosen
    Article
    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33
    Insert
    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
    Use
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    unknown
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    221068-plasmid-map-sequence-id-459203

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  30. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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  31. pCMT-flp

    Plasmid
    #67274
    Purpose
    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis
    Depositor
    Mark Liles
    Article
    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-
    Insert
    tetA, flp, repA101, oriR101
    Use
    Flp recombinase plasmid, temperature sensitive
    Tags
    His-SUMO (flp)
    Expression
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67274-plasmid-map-sequence-id-115886

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  42. pCm N-term SMT3 NikJC199U

    Plasmid
    #174210
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174210-plasmid-map-sequence-id-342456

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  43. pCm C-term SMT3 NikJC199U

    Plasmid
    #174362
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174362-plasmid-map-sequence-id-342846

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  47. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. pGBW-m4136463

    Plasmid
    #149527
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149527-plasmid-map-sequence-id-300219

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Showing: 801 - 850 of 1220 results