Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,940 results for: 338

Showing: 321 - 340 of 5940 results

  4. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Expression
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

    Enlarge

  11. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid
    #214282
    Purpose
    Express split-RhoA fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    RhoA-Q63L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214282-plasmid-map-sequence-id-422245

    Enlarge

  15. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

    Plasmid
    #214283
    Purpose
    Express split-RhoA fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    RhoA-T19N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214283-plasmid-map-sequence-id-422243

    Enlarge

  16. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCS2+TA

    Plasmid
    #49338
    Purpose
    pCS2+ vector with MCS replaced by a TA-cloning linker
    Depositor
    Heather Verkade
    Insert
    TA-cloning linker containing EGFP
    Use
    Gateway compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    49338-plasmid-map-sequence-id-73127

    Enlarge

  18. T4 Lysozyme L46A WT*

    Plasmid
    #18338
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L46A WT*
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  19. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133885-plasmid-map-sequence-id-274419

    Enlarge

  20. p15A-PB

    Plasmid
    #133886
    Purpose
    piggyBac ITR's in low copy number p15A backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133886-plasmid-map-sequence-id-274420

    Enlarge

Showing: 321 - 340 of 5940 results