We narrowed to 2,461 results for: T2A

1. pCXLE-dCas9VP192-T2A-EGFP

   Plasmid

   #69536

   Purpose

   Episomal plasmid encoding dCas9VP192

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTet-O-LHX8-T2A-PuroR

   Plasmid

   #162350

   Purpose

   Lentiviral expression of LHX8 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   LHX8 (LHX8 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

   Plasmid

   #216495

   Purpose

   Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloEKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dCas9-T2A-GFP

   Plasmid

   #193781

   Purpose

   Tricistronic expression of dCas9-EGFP-BSD

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   dead Cas9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   In frame with existing Cas9 promoter

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60657

   Purpose

   Expresses mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   mNaChBacMut-T2A-tdTomato
   

   Expression

   Mammalian

   Mutation

   E191K

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2K-p53DN-T2A-copGFP

   Plasmid

   #109229

   Purpose

   For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   dominant negative p53 (Trp53 Mouse)
   

   Tags

   copGFP

   Expression

   Mammalian

   Mutation

   dominant negative p53

   Promoter

   CAGGS

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX209-neo-HA-Ub-T2A-Tet3G

   Plasmid

   #202436

   Purpose

   Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   Ubiquitin
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLARRYv2-EGFP-T2A-Flpo

   Plasmid

   #233212

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF3BGX-T2A-GAL4-DBD

   Plasmid

   #138394

   Purpose

   RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-GAL4-DBD
   

   Expression

   Insect

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRF1-GW ymScarletI-T2A-mTurquoise2

    Plasmid

    #118441

    Purpose

    Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI-T2A-mTurquoise2
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMy-MouseCTCFbiotag-T2A-mOrange

    Plasmid

    #50564

    Purpose

    Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Inserts

    Ampicilin
    Mouse CTCF Exons
    

    Use

    Retroviral

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pShuttle-CMV-F2A-T2A

    Plasmid

    #62622

    Purpose

    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A

    Depositor

    Zheng-Xu Wang

    Insert

    MCS with F2A and T2A
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mScarletI-T2A-mTq2 pcDNA3.1

    Plasmid

    #230048

    Purpose

    Expresses mScarletI-T2A-mTq2 in human cells

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    mScarletI-T2A-mTq2
    

    Available Since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-CB-copGFP-T2A-iCre

    Plasmid

    #72256

    Purpose

    Express copGFP and iCre under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-iCre
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available Since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAGGS-pac-T2A-iCre (v2)

    Plasmid

    #125821

    Purpose

    Puromycin resistance gene and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAID 1.1 N-T2A-Bsr

    Plasmid

    #105985

    Purpose

    To make an auxin inducible degron mutant

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

    Insert

    T2A-Bsr
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Expression

    Worm

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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