Addgene

We narrowed to 11,595 results for: cel.2;

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pX330A-Prdm14-L3-#2

Plasmid

#171510

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Prdm14-L4-#2

Plasmid

#171512

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPD127 AND Gate, 2 repeats

Plasmid

#138936

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 2 repeats

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Logic 37 AND 158 NOT 43 2 repeats

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA1_SARS-COV-2_ORF3a

Plasmid

#169390

Purpose

Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP

Depositor

Kirill Alexandrov

Insert

SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)

Use

Cell-free expression vector

Tags

His and eGFP

Expression

Mutation

Codon optimized for Saccharomyces cerevisiae

Promoter

T7- CMV/CAG

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_SARS-COV-2_ORF7b

Plasmid

#169394

Purpose

Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP

Depositor

Kirill Alexandrov

Insert

SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)

Use

Cell-free expression vector

Tags

His and eGFP

Expression

Mutation

Codon optimized for Saccharomyces cerevisiae

Promoter

T7- CMV/CAG

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

MMP2

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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Lsg-GBA-1 + 2

Plasmid

#199276

Purpose

nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA nick

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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E47 2/5-pCS2

Plasmid

#20914

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E47 2/5 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
1/2-NORAD-(A)4x

Plasmid

#199208

Purpose

1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/2-NORAD

Use

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-PDZ1-2

Plasmid

#28294

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-298 (deletion of the EB domain aa 2…

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pBS mouse IRS-2

Plasmid

#11372

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
Lsg-LMNA-1 + 2

Plasmid

#199278

Purpose

nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA nick

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #2

Plasmid

#27017

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #2 (NEDD4L Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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E12 2/5-pCS2

Plasmid

#20913

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 2/5 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSuper mouse DGCR8-2

Plasmid

#14773

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only