Addgene

We narrowed to 5,714 results for: cki

Showing: 321 - 340 of 5714 results

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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-Golgi-7

Plasmid

#57722

Purpose

Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Expression

Mutation

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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HP138-puro

Plasmid

#134246

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

Contains Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calreticulin (CALR Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
HP138-neo

Plasmid

#134247

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

Contains Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-NOP56-C3

Plasmid

#134536

Purpose

Expresses YFP-NOP56 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

NOP56 (NOP56 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-RPS5-C1

Plasmid

#134534

Purpose

Expresses YFP-RPS5 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

RPS5 (RPS5 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Fascin-C-10

Plasmid

#57376

Purpose

Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Fascin (FSCN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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th-P2A-EGFP donor

Plasmid

#65562

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-EGFP (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

Plasmid

#132546

Purpose

Expresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TBP6.7 non-targeting gRNA lambda phage

Plasmid

#132547

Purpose

expresses gRNA for TBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA TBP-based CIRTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SLBP non-targeting gRNA lambda phage

Plasmid

#132548

Purpose

expresses gRNA for SLBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA SLBP-based CIRTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Plasmid

#132544

Purpose

Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-LaminB1-10

Plasmid

#57728

Purpose

Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LaminB1 (LMNB1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits