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We narrowed to 4,195 results for: spl

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Showing: 321 - 340 of 4195 results

  4. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Publication
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  5. pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

    Plasmid
    #208692
    Purpose
    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208692-plasmid-map-sequence-id-412344

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  9. pDisplay-GRAB_CRFmut

    Plasmid
    #208656
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208656-plasmid-map-sequence-id-410341

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  10. pDisplay-GRAB_CRF0.5

    Plasmid
    #208654
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208654-plasmid-map-sequence-id-410339

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  14. Spike Display_Part 2 DO

    Plasmid
    #172722
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Publication
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172722-plasmid-map-sequence-id-344857

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  15. USPL1cat-pEYFP_C1

    Plasmid
    #85766
    Purpose
    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion
    Depositor
    Frauke Melchior
    Publication
    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    catalytic domain (aa 212-514)
    Promoter
    CMV
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85766-plasmid-map-sequence-id-169509

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  17. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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Showing: 321 - 340 of 4195 results