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We narrowed to 704 results for: transfection

Showing: 321 - 340 of 704 results

  1. pIhh715bp-Luc

    Plasmid
    #53606
    Purpose
    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pIhh4.5k-Luc

    Plasmid
    #53603
    Purpose
    Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

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  7. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  11. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  12. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  13. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Mikko Taipale, Benjamin Blencowe
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  15. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  16. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

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  17. CAG-Cremyc-2A-GFP

    Plasmid
    #116879
    Purpose
    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.
    Depositor
    Qiang Lu
    Article
    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:
    Insert
    Cremyc-2A-GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    116879-plasmid-map-sequence-id-243398

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  18. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

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  19. pAF123

    Plasmid
    #135921
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    135921-plasmid-map-sequence-id-270083

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Showing: 321 - 340 of 704 results