We narrowed to 838 results for: transfection

1. HA-mios in pRK5

   Plasmid

   #154212

   Purpose

   N-terminal HA-tagged Mios cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   Mios
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   cDNA sequence has been codon optimized using huma…

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RDX-mCherry

   Plasmid

   #214476

   Purpose

   Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   RDX (RDX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Sequence matches cDNA clone MGC:48283 IMAGE:52844…

   Promoter

   CMV

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SEH1L in pRK5

   Plasmid

   #154216

   Purpose

   Untagged SEH1L cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   SEH1L (SEH1L Human)
   

   Tags

   NONE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MPRA Empty Vector

   Plasmid

   #204865

   Purpose

   Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

   Depositor

   Philipp Maass

   Article

   Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-DDK

   Plasmid

   #137764

   Purpose

   Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GFP
   

   Tags

   DDK

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTarget TMEM97-P2A-mCardinal

   Plasmid

   #157747

   Purpose

   Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Promoter

   CMV/TetO2

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS_4xMx PylT C41CA_GFP 150TAG

   Plasmid

   #140017

   Purpose

   Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIhh742bp-Luc

    Plasmid

    #53605

    Purpose

    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIhh1.3K-Luc

    Plasmid

    #53604

    Purpose

    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

13. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDOE11 mas:mTq2 transient

    Plasmid

    #124248

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIhh715bp-Luc

    Plasmid

    #53606

    Purpose

    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBOB-3xFlag-hGpr75-p.Gln234*

    Plasmid

    #236748

    Purpose

    Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    GPR75 (GPR75 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Gln234 to STOP codon, CAA to TAA

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBOB-3xFlag-hGpr75-p.Ala110fs

    Plasmid

    #236747

    Purpose

    Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    GPR75 (GPR75 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Ala110fs, deletion of GCCAGTA

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FHpHG_mTent5a

    Plasmid

    #221439

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5a (Fam46a Mouse)
    

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FHpHG_mTent5c

    Plasmid

    #221440

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c (Fam46c Mouse)
    

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIhh4.5k-Luc

    Plasmid

    #53603

    Purpose

    Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only