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We narrowed to 784 results for: transfection

Showing: 321 - 340 of 784 results

  3. pIhh742bp-Luc

    Plasmid
    #53605
    Purpose
    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 30, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pIhh1.3K-Luc

    Plasmid
    #53604
    Purpose
    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. C211

    Plasmid
    #174360
    Purpose
    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection
    Depositor
    David Bartel
    Article
    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1
    Insert
    PABPC1 (PABPC1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …
    Promoter
    Available since
    Feb. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    174360-plasmid-map-sequence-id-343304

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  8. pIhh715bp-Luc

    Plasmid
    #53606
    Purpose
    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pBOB-3xFlag-hGpr75-p.Gln234*

    Plasmid
    #236748
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Gln234 to STOP codon, CAA to TAA
    Promoter
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    236748-plasmid-map-sequence-id-477901

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  10. pBOB-3xFlag-hGpr75-p.Ala110fs

    Plasmid
    #236747
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Ala110fs, deletion of GCCAGTA
    Promoter
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    236747-plasmid-map-sequence-id-477894

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  13. pIhh4.5k-Luc

    Plasmid
    #53603
    Purpose
    Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Mikko Taipale, Benjamin Blencowe
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  20. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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Showing: 321 - 340 of 784 results