We narrowed to 484 results for: transposase

1. pSL0283 (pTnsABC)

   Plasmid

   #130633

   Purpose

   Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTnsA, VchTnsB, VchTnsC
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAM_Cam

   Plasmid

   #91570

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

   Plasmid

   #223287

   Purpose

   A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
   

   Use

   Synthetic Biology; Piggybac transposase insertabl…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCis GFPuv-SS Himar1 A7

   Plasmid

   #84686

   Purpose

   Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

   Depositor

   Ulrike Munderloh

   Article

   Munderloh et al Intracellular Pathogens II: Rickettsiale

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

   Plasmid

   #136262

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCis mCherry-SS Himar1 A7

   Plasmid

   #84687

   Purpose

   Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

   Depositor

   Ulrike Munderloh

   Article

   Munderloh et al Intracellular Pathogens II: Rickettsiale

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS12: pDonor(AvCAST)

   Plasmid

   #168145

   Purpose

   AvCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST right end (RE)
   AvCAST left end (LE)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS13: pTarget(AvCAST)

    Plasmid

    #168146

    Purpose

    Target plasmid containing AvPSP1 and attachement site for AvCAST.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PAM for AvCAST + AvPSP1
    glmS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS15: pTwinStrep-SUMO-AvTnsB

    Plasmid

    #168148

    Purpose

    AvTnsB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS16: pTwinStrep-SUMO-AvTnsC

    Plasmid

    #168149

    Purpose

    AvTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsC
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMS14: pTwinStrep-SUMO-AvTnsA

    Plasmid

    #168147

    Purpose

    AvTnsA expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsA
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRoCascade_gfp

    Plasmid

    #178771

    Purpose

    Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRoDonor

    Plasmid

    #178773

    Purpose

    Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tol2 8xSTAR-mScarletI. PGK-puro

    Plasmid

    #136266

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP-PH16_RS09790-pcepA-himar-chlorR

    Plasmid

    #238494

    Purpose

    Landing Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

    Depositor

    Farren Isaacs

    Article

    Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BenM/PH16_RS09790

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLP-ParaBAD-pcepA-himar-chlorR

    Plasmid

    #238491

    Purpose

    Landing Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

    Depositor

    Farren Isaacs

    Article

    Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC/ParaBAD

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLP-PH16_RS08125-pcepA-himar-chlorR

    Plasmid

    #238493

    Purpose

    Landing Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

    Depositor

    Farren Isaacs

    Article

    Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NahR/PH16_RS08125

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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