We narrowed to 3,452 results for: ttl

1. pLNCX1 myr Akt1 delta4-129

   Plasmid

   #15989

   Depositor

   Morris Birnbaum

   Article

   Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Delta 4-129

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

2. myc-AP4 Epsilon (V454X)

   Plasmid

   #127624

   Purpose

   N-terminal myc-tagged AP4 epsilon subunit (V454X)

   Depositor

   Josef Kittler

   Article

   Ivankovic et al Autophagy. 2019 May 29:1-17. doi: 10.108

   Insert

   AP-4 complex subunit epsilon-1 (AP4E1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Premature stop codon (V454X)

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB9v2

   Plasmid

   #210029

   Purpose

   Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB9P2

   Plasmid

   #210030

   Purpose

   Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-Miro1 (1-177 fused to 562-618)

   Plasmid

   #127627

   Purpose

   First GTPase domain of human Miro1 fused to C-terminus

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   1-177 fused to 562-618

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Miro1 (412-618)

   Plasmid

   #127628

   Purpose

   GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   412-618 only

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc-Miro2 (lacking TM)

   Plasmid

   #127614

   Purpose

   Human Miro2 with the transmembrane deleted

   Depositor

   Josef Kittler

   Article

   Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

   Insert

   Miro2 (RHOT2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Transmembrane deletion

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Ubiquitin-K11R

    Plasmid

    #121154

    Purpose

    Mammalian expression of HA tagged ubiquitin K11R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K11 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-Ubiquitin-K27R

    Plasmid

    #121155

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K27 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-Ubiquitin-K6R

    Plasmid

    #121153

    Purpose

    Mammalian expression of HA tagged ubiquitin K6R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K6 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-Ubiquitin-K27R-K29R

    Plasmid

    #121156

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R and K29R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K27 and K29 mutated to Rs

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB9P3

    Plasmid

    #210034

    Purpose

    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-Ubiquitin-K11

    Plasmid

    #121152

    Purpose

    Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    All Ks apart from K11 mutated to Rs

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-FLAG-HAND1-T2A-mTagBFP2

    Plasmid

    #223192

    Purpose

    Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    HAND1 (HAND1 Human)
    

    Use

    Promoterless entry vector for gateway cloning

    Tags

    FLAG and T2A-mTagBFP2

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-Gs protein, alpha subunit [N192/12]

    Plasmid

    #190302

    Purpose

    Mammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pP150

    Plasmid

    #113201

    Purpose

    shuttle vector, N-terminal mTurquoise2 tagging

    Depositor

    Rob Kay

    Type

    Empty backbone

    Use

    Dictyostelium discoideum shuttle vector, n-termin…

    Tags

    mTurquoise2

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR_mCherry

    Plasmid

    #139554

    Purpose

    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    mCherry
    

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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