We narrowed to 14,237 results for: cas9 genes

Showing: 8451 - 8500 of 14237 results

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pCMV-hA3A-eBE-Y132D

Plasmid

#113429

Purpose

Expresses hA3A-eBE-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

Plasmid

#75412

Purpose

Module for the expression of the Renilla Luciferase with the silencing suppressor P19

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Renilla / P19

Use

Luciferase and Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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gh113

Plasmid

#106783

Purpose

expression of gRNA targeting B4GALNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALNT1 (B4GALNT1 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Promoter

U6

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ256

Plasmid

#124312

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA1-BE3

Plasmid

#113418

Purpose

Expresses hA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh266

Plasmid

#106865

Purpose

expression of gRNA targeting ALG12

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG12 (ALG12 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh152

Plasmid

#106822

Purpose

expression of gRNA targeting GLT8D2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GLT8D2 (GLT8D2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE GSG1l-GFP KI

Plasmid

#131492

Purpose

Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gsg1l Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL062

Plasmid

#124894

Purpose

For genome editing in plant by SpCas9 wild type

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-CS-154

Plasmid

#166230

Purpose

lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

Depositor

Nozomu Yachie

Article

Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

Inserts

U6-gRNA EGFP targetting
nCas9-PmCDA1-UGI-2A-mCherry

Use

CRISPR and Lentiviral

Promoter

CMV promoter and Human U6

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-cAICDA-BE3

Plasmid

#113422

Purpose

Expresses cAICDA-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA-TAZ

Plasmid

#86688

Purpose

Expresses a gRNA targeted to human TAZ

Depositor

William Pu

Article

Wang et al Nat Protoc. 2017 Jan;12(1):88-103. doi:

Insert

U6-gRNA-TAZ (TAFAZZIN Human)

Use

CRISPR

Promoter

U6

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAP1716-1

Plasmid

#105245

Purpose

human eGFP with homology arms for Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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gh126

Plasmid

#106796

Purpose

expression of gRNA targeting ST8SIA6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA6 (ST8SIA6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh125

Plasmid

#106795

Purpose

expression of gRNA targeting ST8SIA5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA5 (ST8SIA5 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS663-pMCSG7-AcrIIC4Hpa

Plasmid

#113440

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Tags

6xHis

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS664-pMCSG7-AcrIIC5Smu

Plasmid

#113441

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Tags

6xHis

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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gh83

Plasmid

#106743

Purpose

expression of gRNA targeting MGAT5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT5 (MGAT5 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL065

Plasmid

#124896

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gh60

Plasmid

#106720

Purpose

expression of gRNA targeting B3GAT3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GAT3 (B3GAT3 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ255

Plasmid

#124310

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A-UGI

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE C9orf4-GFP KI

Plasmid

#131472

Purpose

Endogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Frrs1l Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

Plasmid

#68346

Purpose

Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

Depositor

Jannik Elverløv-Jakobsen

Article

Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

Inserts

3xgRNA;PTENA, p53B,SMAD4A
FlPO

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

CAG and U6

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
gh142

Plasmid

#106812

Purpose

expression of gRNA targeting EOGT

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EOGT (EOGT Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_046

Plasmid

#123976

Purpose

Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hThal5.10-2_hg18 CAS9 Destination - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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gh5

Plasmid

#106689

Purpose

expression of gRNA targeting GALNT5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT5 (GALNT5 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh14

Plasmid

#106698

Purpose

expression of gRNA targeting GALNT14

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT14 (GALNT14 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh13

Plasmid

#106694

Purpose

expression of gRNA targeting GALNT13

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT13 (GALNT13 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh116

Plasmid

#106786

Purpose

expression of gRNA targeting GCNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT2 (GCNT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh260

Plasmid

#106851

Purpose

expression of gRNA targeting ALG6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG6 (ALG6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh261

Plasmid

#106852

Purpose

expression of gRNA targeting ALG8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG8 (ALG8 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh1

Plasmid

#106682

Purpose

expression of gRNA targeting GALNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT1 (GALNT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ255-x3.7

Plasmid

#124311

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A_x3.7-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ256-x3.7

Plasmid

#124313

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A_x3.7-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh66

Plasmid

#106737

Purpose

expression of gRNA targeting B4GALT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALT6 (B4GALT6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-BE3-C101S

Plasmid

#113426

Purpose

Expresses hA3A-BE3-C101S in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-C101S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_C101S

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-BE3-C106S

Plasmid

#113427

Purpose

Expresses hA3A-BE3-C106S in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-C106S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_C106S

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS48

Plasmid

#241839

Purpose

Cas9-gRNA construct targeting the UBE3A locus

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

Cas9-gRNA (UBE3A )

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 14,237 results for: cas9 genes