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We narrowed to 11,999 results for: SHA

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Showing: 3381 - 3400 of 11999 results

  1. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51762-plasmid-map-sequence-id-79380

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  6. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid
    #120513
    Purpose
    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC Thr58Ala (MYC Human)
    Use
    Lentiviral
    Mutation
    Point mutation changing Threonine to Alanine at a…
    Promoter
    EF1a
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120513-plasmid-map-sequence-id-234974

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  7. pNK115

    Plasmid
    #219745
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219745-plasmid-map-sequence-id-450697

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  8. pNK116

    Plasmid
    #219742
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219742-plasmid-map-sequence-id-437105

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  10. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. lentiCRISPR - EGFP sgRNA 6

    Plasmid
    #51765
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51765-plasmid-map-sequence-id-79389

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  14. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid
    #120506
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC H84NLS (MYC Human)
    Use
    Lentiviral
    Mutation
    Deletion of nuclear localization signal sequence
    Promoter
    EF1a
    Available Since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120506-plasmid-map-sequence-id-234926

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  15. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid
    #120514
    Purpose
    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC Ser62Ala (MYC Human)
    Use
    Lentiviral
    Mutation
    Point mutation changing Serine to Alanine at amin…
    Promoter
    EF1a
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120514-plasmid-map-sequence-id-234976

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  19. pOPINF Ta-sro1 WWE-PARP

    Plasmid
    #192550
    Purpose
    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)
    Depositor
    Lennart Wirthmueller
    Article
    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d
    Insert
    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    Tags
    His6, 3C protease cleavage site
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available Since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    192550-plasmid-map-sequence-id-378819

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Showing: 3381 - 3400 of 11999 results