We narrowed to 5,896 results for: dna plasmids

1. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hAID-BE3 (pRZ352)

   Plasmid

   #131316

   Purpose

   CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-short Cre-on G/W

   Plasmid

   #86949

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. JA740

   Plasmid

   #49939

   Purpose

   Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD GFP (TET1 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ889

   Plasmid

   #84826

   Purpose

   Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   smu-1 (smu-1 Nematode)
   

   Tags

   GFP

   Expression

   Bacterial and Worm

   Promoter

   Psmu-1

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

   Plasmid

   #163644

   Purpose

   Makes a lentivirus that expresses mNeonGreen and Cas9.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   mNeonGreen-P2A-Cas9
   

   Use

   Lentiviral

   Tags

   mNeonGreen-P2A

   Promoter

   EFS

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. JA524

   Plasmid

   #49938

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD GFP (TET1 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

9. sTR056

   Plasmid

   #177369

   Purpose

   Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SL537

    Plasmid

    #49945

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Sox2-SE-RGM-eGFP targeting vector

    Plasmid

    #133045

    Purpose

    Targeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMR

    Depositor

    Rudolf Jaenisch

    Article

    Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

    Insert

    Sox2-SE-5arm-RGM-Sox2-SE-3arm
    

    Use

    Mouse Targeting

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

    Plasmid

    #106434

    Purpose

    pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Plant

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SL536

    Plasmid

    #49942

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

14. SL534

    Plasmid

    #49940

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-1 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. SL535

    Plasmid

    #49941

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-2 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. AL05_pDonor.DMD

    Plasmid

    #100284

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AC62_pDonor.DMD.TS.DR

    Plasmid

    #100288

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBQ10::ZF108_3xFLAG_TET1cd

    Plasmid

    #106432

    Purpose

    pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    pUBQ10::ZF108_3xFLAG_TET1cd (TET1 Mustard Weed, Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SL539

    Plasmid

    #49948

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pPSmF

    Plasmid

    #226912

    Purpose

    Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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