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pWS5394-CRISPRai-MET17

Plasmid

#182833

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pMA4854

Plasmid

#184849

Purpose

Coexpression of PhiC31o recombinase and EGFP

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Inserts

EGFP
PhiC31o

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and RSV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Use

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD6iresGFP

Plasmid

#45027

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD6 (Neurod6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

Plasmid

#216382

Purpose

Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

6xHis-3C-splitGFP11

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Use

Tags

Linker and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-SNX3

Plasmid

#31573

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sorting nexin 3 (SNX3 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWS5392-CRISPRai-HIS3

Plasmid

#182831

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HIS3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5393-CRISPRai-TRP1

Plasmid

#182832

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5395-CRISPRai-LYS2

Plasmid

#182834

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5391-CRISPRai-LEU2

Plasmid

#182830

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-RAB17

Plasmid

#34812

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

RAB17 (RAB17 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p6647 MSCV-P C-Flag HA 2aE7

Plasmid

#35137

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV2a E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6656 MSCV-P C-FlagHA 25E7-Kozak

Plasmid

#35022

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Use

Tags

eGFP

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7025

Plasmid

#128810

Purpose

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-TrioN

Plasmid

#129621

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

N-terminal part of TRIO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Use

Tags

TN-XXL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Myo 19-GFP

Plasmid

#134988

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-perox-tagRFP

Plasmid

#185600

Purpose

Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-tagRFP-T

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDX_init containing Sb-loop

Plasmid

#132697

Purpose

pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop

Use

M13 phagmid vector

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-PDESr

Plasmid

#61207

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Roger Tsien, Erik Rodriguez

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS puro miR30-IRF1

Plasmid

#117156

Purpose

Target site TTGCTCTTAGCATCTCGGCTG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR TCF4

Plasmid

#16514

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-APr

Plasmid

#61183

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-ACTLr

Plasmid

#61194

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-MTUBr

Plasmid

#61197

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6801 - 6840 of 25958 results

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