We narrowed to 12,555 results for: LIC

1. pGEX6P2-MDMX (1-120)

   Plasmid

   #62065

   Purpose

   Expression of human MDM2 (1-120) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 1-120

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

   Plasmid

   #22023

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450A.Rac1 s…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

   Plasmid

   #18865

   Depositor

   Tobias Meyer

   Article

   Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

   Insert

   Stromal interaction molecule 1 (STIM1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   3 mutations (A79K, N80D, D82G) in the EF hand of …

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Human, Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP C1-Eps8 ∆bund

   Plasmid

   #74889

   Purpose

   mammalian expression of the bundling activity mutant Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L757A, K759A

   Promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

   Plasmid

   #208972

   Purpose

   Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MigR1-AE9aP

   Plasmid

   #12434

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Nat Med. 2006 Aug . 12(8):945-9.

   Insert

   AML1-ETO9a as in t(8;21) patients (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AML1-ETO9a cDNA as in t(8;21) patients

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

   Plasmid

   #157702

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR223_STK11_WT

   Plasmid

   #82320

   Purpose

   Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   STK11 (STK11 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid

    #169914

    Purpose

    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_SGK1_WT

    Plasmid

    #81834

    Purpose

    Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK1 (SGK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-M-Flag-BAP1-G185R

    Plasmid

    #125849

    Purpose

    express mutant BAP1-G185R

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    G185R

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4-His-Max-A-PQBP1-Y65C

    Plasmid

    #25803

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Apr 21. ():.

    Insert

    PQBP1 with Y65C mutation (PQBP1 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Tyrosine 65 within the WW domain of PQBP1 is chan…

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223_WNT2_WT

    Plasmid

    #81775

    Purpose

    Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT2 (WNT2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

    Plasmid

    #145842

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. scAAV-TTR-mFgf15

    Plasmid

    #190594

    Purpose

    AAV construct containing Fgf15 cds under control of TTR promoter

    Depositor

    Roel Nusse

    Article

    Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

    Insert

    Fgf15 (Fgf15 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ttr

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-M-Flag-BAP1-E31A

    Plasmid

    #125843

    Purpose

    express mutant BAP1-E31A

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    E31A

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-M-Flag-BAP1-S10T

    Plasmid

    #125842

    Purpose

    express mutant BAP1-S10T

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    S10T

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223_NFKBIB_WT

    Plasmid

    #82219

    Purpose

    Gateway Donor vector containing NFKBIB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NFKBIB (NFKBIB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid

    #157701

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pDONR223_SV2C_WT

    Plasmid

    #81924

    Purpose

    Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SV2C (SV2C Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F346S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pLVX-M-Flag-BAP1-L49V

    Plasmid

    #125844

    Purpose

    express mutant BAP1-L49V

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    L49V

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFLAG-EEF1D (pcDNA 3.1+) LG3-1

    Plasmid

    #37364

    Depositor

    David Litchfield

    Article

    Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

    Insert

    EEF1D (EEF1D Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

26. lentiCRISPR v2-Nr4a1sgRNA1

    Plasmid

    #160945

    Purpose

    Guide RNA 1 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 1 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag‐mTim3 (del.Cyto)

    Plasmid

    #49209

    Purpose

    expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (del.Cyto) (Havcr2 Mouse)
    

    Use

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    cytosolic domain deleted, no Tim3 signal sequence…

    Promoter

    Ef1a

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmiR-report-MYB-WT

    Plasmid

    #141114

    Purpose

    Reporter plasmid of WT MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    CMYB (MYB Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_PKIA_WT

    Plasmid

    #82153

    Purpose

    Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PKIA (PKIA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-M-Flag-BAP1-F170C

    Plasmid

    #125848

    Purpose

    express mutant BAP1-F170C

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    F170C

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLVX-M-Flag-BAP1-G109V

    Plasmid

    #125846

    Purpose

    express mutant BAP1-G109V

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    G109V

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 (-) hYAP 1-2gamma

    Plasmid

    #124145

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. actin CP alpha 2 beta 2

    Plasmid

    #13452

    Depositor

    John Cooper

    Article

    Bhattacharya et al J Biol Chem. 2006 Oct 13. 281(41):31021-

    Insert

    capping protein alpha 2 beta 2 (mouse) (Capzb, Capza2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

35. sgSf3b1(T1)

    Plasmid

    #90424

    Purpose

    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700

    Depositor

    Alex Minella

    Article

    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Insert

    mus Sf3b1 gRNA (Sf3b1 Mouse)
    

    Use

    CRISPR; Guiderna encoding for gene editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

    Plasmid

    #20174

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    3 mutations (K83A,D84N,G86D) in the EF hand of ST…

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETM6-3F2-mCherry

    Plasmid

    #73418

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 (-) hYAP 1-1alpha

    Plasmid

    #124139

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223_TPTE_WT

    Plasmid

    #81867

    Purpose

    Gateway Donor vector containing TPTE , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TPTE (TPTE Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    L452P

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmiR-report-MYB-mut

    Plasmid

    #141115

    Purpose

    Reporter plasmid of mutant MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    A to T mutations at m6A sites

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. G30, Cdc20-mCherry-AID tagging

    Plasmid

    #130270

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDONR223_ROGDI_WT

    Plasmid

    #81814

    Purpose

    Gateway Donor vector containing ROGDI , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ROGDI (ROGDI Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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43. pLV-Hyg-Snrnp40-CRISPR-resistant

    Plasmid

    #134251

    Purpose

    Lentivector encoding CRISPR-resistant Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated coding sequence “gataactatgcgacgttgaa” to…

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA3.1_SNORD27h_2ntmut

    Plasmid

    #73067

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (E2F7 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    2 nt mutation in AS box that binds E2F7 pre-mRNA

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

45. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. pcDNA3.1_hE2F7mg_PP7-tag

    Plasmid

    #73078

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    5'-PP7-tag RNA

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDONR223_OTUD6A_WT

    Plasmid

    #81813

    Purpose

    Gateway Donor vector containing OTUD6A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pCMV6-Entry IL36RN S113L

    Plasmid

    #58321

    Purpose

    entry vector for human IL36RN S113L mutant

    Depositor

    Francesca Capon

    Article

    Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

    Insert

    IL36RN (IL36RN Human)
    

    Use

    Entry vector

    Tags

    myc-DDK

    Expression

    Mutation

    S113L: c.338C>T, p.Ser113Leu

    Promoter

    CMV

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

50. pBABE (hygro) hYAP 1-1delta

    Plasmid

    #124150

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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