We narrowed to 10,893 results for: ENA;

1. pMOD_A1610

   Plasmid

   #91038

   Purpose

   Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-TaCas9_D10A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   ZmUbi

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-Blunt II-TOPO-PTPRD

   Plasmid

   #29705

   Depositor

   Todd Waldman

   Article

   Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

   Insert

   Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   none

   Promoter

   Available Since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pSilencer2.1-U6-NL1F35

   Plasmid

   #190830

   Purpose

   For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   NL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMXs_FLAG-ODC1

   Plasmid

   #133256

   Purpose

   Express FLAG-tagged ODC1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   ODC1 (ODC1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Promoter

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEY79

   Plasmid

   #191074

   Purpose

   flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-12 (flp-12 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY62

   Plasmid

   #191020

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5 (acr-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1102

   Plasmid

   #119246

   Purpose

   knockdown phzA1 in P. aeruginosa

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA phzA1 (P. aeruginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP1103

   Plasmid

   #119247

   Purpose

   knockdown phzM in P. aeruginosa

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA phzM (P. aeruginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTaCas9_6

   Plasmid

   #91169

   Purpose

   Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRRNA-G20-R20

    Plasmid

    #101822

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1055

    Plasmid

    #119242

    Purpose

    construction intermediate

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DCT.VV.Orf137

    Plasmid

    #12825

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf137
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. E0030 (YFP)_CD

    Plasmid

    #66031

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPKm-118

    Plasmid

    #90491

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    pFR Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS5

    Plasmid

    #55750

    Purpose

    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Residues 59-264 of KanR

    Promoter

    T7-lac

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry2-sgC9cen

    Plasmid

    #198326

    Purpose

    Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    Chr9-CEN guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Empty Anchor Control

    Plasmid

    #127665

    Purpose

    Encodes for puromycin resistance expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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