We narrowed to 11,102 results for: ENA;

1. pJMP1106

   Plasmid

   #119249

   Purpose

   construction intermediate

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. DCT.VV.Orf221

   Plasmid

   #12909

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf221
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pSCIP-hTRAC

   Plasmid

   #154094

   Purpose

   Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA and BAIT targeting human TRAC locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAAVS1 Insulator ObLiGaRe/EPB71

   Plasmid

   #90018

   Purpose

   Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

   Depositor

   Eric-Paul Bennett

   Article

   Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

   Insert

   MCS flanked by insulator and inverted ZFN binding sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK RagA-RagC

   Plasmid

   #99649

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   10x His tag, Poly arginine linker, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-Neuroserpin

   Plasmid

   #58259

   Purpose

   Retroviral vector for expressing Neuroserpin in mammalian cells

   Depositor

   Joan Massague

   Article

   Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

   Insert

   Neuroserpin (SERPINI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CaMKIIa-ChRger1-TS-EYFP

   Plasmid

   #127244

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC027.306

   Plasmid

   #171149

   Purpose

   Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-T2-NLuc

    Plasmid

    #135938

    Purpose

    Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAtCas9-dead_2

    Plasmid

    #91166

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS1088

    Plasmid

    #80159

    Purpose

    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

    Depositor

    Marcelo Sousa

    Article

    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

    Insert

    HisSUMO-Cys-4xNuG2-Ald
    

    Use

    Tags

    HisSUMO

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTaCas9-H840A_6

    Plasmid

    #91173

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS7: pCascade(AvCAST)_entry

    Plasmid

    #168140

    Purpose

    Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pChlr-4SYR

    Plasmid

    #52158

    Purpose

    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-HA-RIPK3 K55R

    Plasmid

    #78807

    Purpose

    To overexpress RIPK3 K55R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmCup_C

    Plasmid

    #146041

    Purpose

    Insect Expression of DmCup

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmCup (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    P418S

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-RIPK3 S199A

    Plasmid

    #78806

    Purpose

    To overexpress RIPK3 S199A in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S199A

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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