We narrowed to 5,382 results for: kras

1. pRK5-mEGFP-PHOX2B-MUT

   Plasmid

   #194577

   Purpose

   Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   PHOX2B (PHOX2B Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   S207Afs*102

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-FLAG-SHLD2

   Plasmid

   #114117

   Purpose

   Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pYX233-b2delta-DHFR

   Plasmid

   #163760

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2delta-DHFR (Dhfr Mouse, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-eGFP-FHA-SHLD2

   Plasmid

   #114121

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   RNF8 FHA domain (aa 2-160) and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pαH-S-GSAS-OMICRON.BA.2-RBD

   Plasmid

   #184830

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-mEGFP-RAX-WT

   Plasmid

   #194572

   Purpose

   Mammalian Expression of mEGFP-RAX WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   RAX (RAX Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_N-DYK_MTHFD1-NLS

   Plasmid

   #133023

   Purpose

   NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

   Depositor

   Stefan Kubicek

   Article

   Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pαH-S-GSAS-OMICRON.BA.1-RBD

   Plasmid

   #184831

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCW-eGFP-SHLD2-m1

   Plasmid

   #114120

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (m1 mutant) (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   W489A, F494A, W495A mutations introduced

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCW-eGFP-FHA

    Plasmid

    #114129

    Purpose

    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 2-160

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pRK5-mEGFP-RAX-MUT

    Plasmid

    #194573

    Purpose

    Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    RAX (RAX Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    S222Rfs*63

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-mEGFP-MYOD1-MUT

    Plasmid

    #194571

    Purpose

    Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    R188Pfs*90

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3_N-DYK_MTHFD1-NES

    Plasmid

    #133022

    Purpose

    NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGLUE-SHLD2

    Plasmid

    #114118

    Purpose

    Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Tags

    HA tag, Streptavidin-binding tag (Streptag), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. TMEM230-M4/X184PG-IRES(isoform 2)

    Plasmid

    #99568

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M4/X184PG

    Promoter

    CMV

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TMEM230-M3/Y92C-IRES(isoform 2)

    Plasmid

    #99567

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M3/Y92C

    Promoter

    CMV

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMEM230-M2/X184W-IRES(isoform 2)

    Plasmid

    #99566

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2/X184W

    Promoter

    CMV

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TMEM230-M1/R141L-IRES(isoform 2)

    Plasmid

    #99565

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1/R141L

    Promoter

    CMV

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET3a-SF-LMNC-TS

    Plasmid

    #169374

    Purpose

    E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    LMNA residues 31-542 (LMNA Human)
    

    Use

    Tags

    Split-FlAsH Tag and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-30 (unstructured head domain…

    Promoter

    Native pET3a promotor

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TMEM230-M2/X184W-IRES(isoform 1)

    Plasmid

    #99560

    Purpose

    mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2/X184W

    Promoter

    CMV

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge