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pHPRT-DRGFP

Plasmid

#26476

Purpose

DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

Insert

pHPRT-DRGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pSFV3

Plasmid

#92072

Purpose

Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Type

Empty backbone

Use

Empty expression plasmid, for run-off transcripti…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Myo1E-pmAppleC1

Plasmid

#27698

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Myo1E (Myo1e Mouse)

Tags

mApple

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pET28a SV40 ST full length

Plasmid

#132636

Purpose

expresses SV40 ST full length in E. coli

Depositor

Wenqing Xu

Article

Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

Insert

SV40 small t antigen

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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QBEmax

Plasmid

#238001

Purpose

Expresses the QBEmax base editor in mammalian cells

Depositor

Kevin Zhao

Article

Hu et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

QBEmax base editor

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP

Plasmid

#89684

Purpose

pCAG promoter driving EGFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCiPS

Plasmid

#60011

Purpose

Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Pol

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mtagBFP

Plasmid

#89685

Purpose

pCAG promoter driving mtagBFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-RPA194

Plasmid

#17660

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA194 (Polr1a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pLenti SpBsmBI sgRNA Puro

Plasmid

#62207

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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p11L1w

Plasmid

#37330

Depositor

John Schiller

Article

Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

Insert

HPV11 L1

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCiES

Plasmid

#60010

Purpose

Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Env (foamy virus)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcS-mCherry-C1C2

Plasmid

#178425

Purpose

Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

mCherry

Expression

Mammalian

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5 (w71-3)

Plasmid

#17425

Purpose

Gateway entry vector for N-term V5 tag, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltTEVp-40-mCherry

Plasmid

#234064

Purpose

Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltTEV-40-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMV_DUGV_GP

Plasmid

#207268

Purpose

Mammalian expression of Dugbe virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DUGV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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DECOR

Plasmid

#219545

Purpose

Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

DECOR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) -myc-OGA(GS-544)C181

Plasmid

#193996

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-mEGFP

Plasmid

#127813

Purpose

Hetero-dimer mCherry-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-iRFP720

Plasmid

#89687

Purpose

pCAG promoter driving iRFP720

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT miniAID-EGFP

Plasmid

#101713

Purpose

For amplification of a miniAID-EGFP for C-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

miniAID-EGFP

Tags

miniAID-EGFP

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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PB-U6insert

Plasmid

#104536

Purpose

A piggybac vector with a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCS4092

Plasmid

#131741

Purpose

Theophylline switch Theo-CAUAA

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Theophylline switch Theo-CAUAA

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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