We narrowed to 7,278 results for: 11

1. pSuper.Retro.Puro-WDR5 shRNA 4

   Plasmid

   #59977

   Purpose

   Knockdown of WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 shRNA
   

   Use

   Retroviral

   Available Since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRLCon1-3180

   Plasmid

   #31445

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

   Insert

   HNF4A 3'UTR (long) (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3046(gRNA XI-5)

   Plasmid

   #73288

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3044(gRNA XI-2)

   Plasmid

   #73286

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3048(gRNA XII-2)

   Plasmid

   #73290

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA GAL4 CREB 1-341 M1

   Plasmid

   #22973

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

   Insert

   CREB (Creb1 GAL4-fusion, Rat)
   

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   Changed Serine 133 to Alanine of CREB

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1-shPrc1

   Plasmid

   #160959

   Purpose

   Prc1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Prc1 shRNA (Prc1 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cx26-sfGFP

   Plasmid

   #69026

   Purpose

   Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx26 (Gjb2 Rat)
   

   Tags

   non-monomerized superfolder GFP (V206)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK6_016

    Plasmid

    #124058

    Purpose

    Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    WPRE (extended)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATF4 15: 5’ATF4.uORF1stopm1.GFP

    Plasmid

    #21862

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Stop codon for uORF1 converted to UCG (see author…

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSuper.Retro.Puro-PKN1 shRNA 4

    Plasmid

    #59978

    Purpose

    Knockdown of PKN1

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    PKN1 shRNA
    

    Use

    Retroviral

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pG418R-MCS-QF-MiniWhite

    Plasmid

    #165902

    Purpose

    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-G protein Gamma 5

    Plasmid

    #42200

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    Gamma 5 (Gng5 Rat)
    

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

16. MTK2_024

    Plasmid

    #123716

    Purpose

    Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pPGK1c
    

    Expression

    Mammalian

    Mutation

    Crippled Kozac sequence

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. M72-IRES-tauRFP2-ACNF TV

    Plasmid

    #15512

    Depositor

    Peter Mombaerts

    Article

    Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

    Insert

    M72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)
    

    Use

    Mouse Targeting

    Mutation

    Targeting vector to insert IRES-tauRFP2-ACNF imme…

    Available Since

    Aug. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

18. (E6) CMV-p18 C/EBPalpha

    Plasmid

    #25881

    Depositor

    Ormond MacDougald

    Article

    Erickson et al J Biol Chem. 2001 May 11. 276(19):16348-

    Insert

    p18 C/EBPalpha (Cebpa Mouse)
    

    Expression

    Mammalian

    Mutation

    N-terminal truncation, contains only CR4 and bZIP…

    Available Since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pWay21-MKP-1L16A/L17A

    Plasmid

    #13478

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1L16A/L17A (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 16 to Alanine; changed Leucine 1…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

20. hSTARR-seq_OCT4 3UTR vector

    Plasmid

    #99295

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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