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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 3421 - 3440 of 11800 results

  1. FB009

    Plasmid
    #119707
    Purpose
    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC::nptII::Ttub
    Use
    Synthetic Biology
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119707-plasmid-map-sequence-id-236967

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  2. pcDNA3.1-nAChr-Alpha6

    Plasmid
    #48812
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    48812-plasmid-map-sequence-id-363332

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  3. TMED1-bio-His

    Plasmid
    #51832
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED1 (TMED1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51832-plasmid-map-sequence-id-79669

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  4. TMED4-bio-His

    Plasmid
    #51854
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED4 (TMED4 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51854-plasmid-map-sequence-id-79741

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  10. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InPAkt
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  11. pcDNA3-Lyso-InPAkt

    Plasmid
    #184053
    Purpose
    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-InPAkt
    Tags
    Lysosome-associated membrane protein 1 (LAMP1).
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184053-plasmid-map-sequence-id-363221

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  13. hsp70l: hAPOB48-mCherry.

    Plasmid
    #140639
    Purpose
    Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.
    Depositor
    Steven Farber
    Publication
    Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119
    Inserts
    hsp70l (hsp70l Zebrafish)
    APOB (APOB Human)
    Use
    Zebrafish expression
    Tags
    mCherry
    Mutation
    APOB48: amino acid 1-2180
    Promoter
    hsp70l
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140639-plasmid-map-sequence-id-308799

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  17. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid
    #188994
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Publication
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188994-plasmid-map-sequence-id-371647

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  18. mCherry-p62-LIR-mutant (LIRAAAA)

    Plasmid
    #187981
    Purpose
    Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    DDDW335-338AAAA (LIR mutant), silent mutation siR…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187981-plasmid-map-sequence-id-374445

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  19. mcherry p62 delta UBA silent

    Plasmid
    #187984
    Purpose
    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lacking UBA domain (aa389-434) but it has the sil…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187984-plasmid-map-sequence-id-374443

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Showing: 3421 - 3440 of 11800 results