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pMM553

Plasmid

#68543

Purpose

NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Publication

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

Plasmid

#32479

Purpose

Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F-GlyR-IRES-GFP

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMy-MouseCTCFbiotag-T2A-mOrange

Plasmid

#50564

Purpose

Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

Depositor

Rafael Casellas

Publication

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Inserts

Ampicilin
Mouse CTCF Exons

Use

Retroviral

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-Trastuzumab-IgA2/κ

Plasmid

#61889

Purpose

Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pN1-PinkyCaMP

Plasmid

#232861

Purpose

A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

Depositor

Olivia Masseck

Publication

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Publication

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Expression

Bacterial

Promoter

J23119

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Publication

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAIP hIL4 co

Plasmid

#74169

Purpose

Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL4 (IL4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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LAMP1-SEP-mRuby3

Plasmid

#200939

Purpose

endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Publication

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

LAMP1 (LAMP1 Human)
SEP-mRuby3

Use

Lentiviral

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Publication

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-Luc-mCherry-puro

Plasmid

#183685

Purpose

Lentiviral vector expressing luciferase and mCherry.

Depositor

Andrew Kung

Publication

Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

Insert

Luc-E2A-mCherry-T2A-PuroR

Use

Lentiviral

Promoter

Human Ubiquitin C (UbC) promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-mCD8

Plasmid

#162747

Purpose

NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCT-tRNA

Plasmid

#133813

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

Depositor

Geraldine Butler

Publication

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter

Use

CRISPR

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIW601-KmCRISPR

Plasmid

#98907

Purpose

K. marxianus CRISPR Plasmid for sgRNA cloning

Depositor

Ian Wheeldon

Publication

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Publication

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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