We narrowed to 8,371 results for: BLI;
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Plasmid#236050PurposeMammalian expression vector with myc-tagged mouse Grhl2 coding sequenceDepositorInsertGrhl2 (Grhl2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IARS2-tb
Plasmid#232340PurposeExpresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cellsDepositorInsertIARS2 (IARS2 Human)
UseTagsExpressionMammalianMutation5 synonymous mutations in the siRNA (ACUUGCAGUCAU…PromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-shortSUMO1
Plasmid#228951Purposeexpress a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimizedDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationdeleted amino acids 1-17PromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RANGAP1-CTD
Plasmid#228947Purposeexpress the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimizedDepositorInsertRANGAP1-CTD (RANGAP1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationcontains aa 418-587 onlyPromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Mex3a_mutRING_t2a_mCherry
Plasmid#223245PurposeExpression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherryDepositorInsertMex3a (Mex3a Mouse)
UseTagsmCherryExpressionMammalianMutation4 point mutations in RING Domain: Cys 484 Ala; Hi…PromoterCMVAvailable sinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-IFIT5_K197R-VA
Plasmid#191223PurposeLentiviral expression of human IFIT5_K197R in mammalian cellsDepositorInsertinterferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
UseLentiviralTagsVA tagExpressionMammalianMutationchanged Lysine 197 to Arginine (K197R)PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA
Plasmid#191217PurposeLentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cellsDepositorInsertSPIKE_SARS2_Alpha (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationDel 69-70 Del 144 N501Y A570D D614G P681H T716I S…PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA
Plasmid#191218PurposeLentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cellsDepositorInsertSPIKE_SARS2_Beta (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationD80A D215G (Del 241-243) K417N E484K N501Y D614G …PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA
Plasmid#191219PurposeLentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cellsDepositorInsertSPIKE_SARS2_Delta (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationT19R 156del 157del R158G L452R T478K D614G P681R …PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA
Plasmid#191220PurposeLentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cellsDepositorInsertSPIKE_SARS2_Lambda (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationG75V T76I Del 246-252 L452Q F490S D614G T859NPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-IFIT5_K160R-VA
Plasmid#191222PurposeLentiviral expression of human IFIT5_K160R in mammalian cellsDepositorInsertinterferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
UseLentiviralTagsVA tagExpressionMammalianMutationchanged Lysine 160 to Arginine (K160R)PromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-HiBiT
Plasmid#191212PurposeTransient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assayDepositorInsertSARS-CoV-2 spike_WT (S SARS CoV-2)
UseTagsHiBiT (high affinity small nanoluciferase fragmen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti Puro HA-Ubiquitin-K48
Plasmid#236290PurposeLentiviral vector expressing HA-tagged ubiquitin K48 mutantDepositorInsertUbiquitin K48 (UBB Human)
UseLentiviralTagsHAExpressionMutationSingle ubiquitin coding sequence containing K48 m…PromoterCMVAvailable sinceApril 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti Puro HA-Ubiquitin-K63
Plasmid#236291PurposeLentiviral vector expressing HA-tagged ubiquitin K63 mutantDepositorInsertUbiquitin K63 (UBB Human)
UseLentiviralTagsHAExpressionMutationSingle ubiquitin coding sequence containing K63 m…PromoterCMVAvailable sinceApril 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR
Plasmid#216664PurposeExpressed Gal4-inducible ETV2 and BFP, with hygromycin resistanceDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-TUBG2
Plasmid#229855PurposeTUBG2DepositorInsertTUBG2 (TUBG2 Human)
UseTagsFlagExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI-mHP1a
Plasmid#232637Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alphaDepositorInsertHaloTag-LacI-mHP1a (Cbx5 Synthetic, Mouse)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif1A-EGFP-SNAP (Kif1a Rat)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Galt1-HT7-mGold
Plasmid#230027PurposeExpressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.DepositorInsertGalt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
UseTagsHaloTag,mGoldExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHOSPHO2-TEV-Twin-Strep
Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PHOSPHO2-TEV-Twin-Strep
Plasmid#222017PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coliDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionBacterialMutationdeleted stop codon (TAA)Promoterlac promoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-167:CDH5-mEGFP
Plasmid#217425PurposeHomology arms and mEGFP sequence for C-terminus tagging of human cadherin 5DepositorInsertCDH5 Homology Arms with mEGFP (CDH5 Human)
UseCRISPR; Donor templateTagsmEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-VP64-2A-mCherryBSD
Plasmid#225695PurposeLentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinRDepositorInsertrTetR-VP64
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-KRAB-2A-mTurquoise
Plasmid#225690PurposeLentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLSDepositorInsertrTetR-ZNF10 KRAB
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)
Plasmid#225442PurposeBeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.DepositorInsertLIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)
Plasmid#225441PurposeModule for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInserthygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
Plasmid#221352PurposeMammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.DepositorInsertAnti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
Plasmid#221353PurposeMammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC; to be used with Anti-GCN4 [IPI-C11L34] light chain (Plasmid 221354) to make the antibody.DepositorInsertAnti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
Plasmid#221351PurposeMammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.DepositorInsertAnti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald
Plasmid#214469PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCF4 - P381L in pENTR
Plasmid#216570Purposegateway cloning donor vector containing TCF4 ASD mutationDepositorInsertTCF4 (TCF4 Human)
UseGateway entry plasmidTagsExpressionMutationP381LPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCF4 - D424N in pENTR
Plasmid#216571Purposegateway cloning donor vector containing TCF4 ASD mutationDepositorInsertTCF4 (TCF4 Human)
UseGateway entry plasmidTagsExpressionMutationD424NPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-APP-CTF
Plasmid#181732PurposeGalactose inducible expression of APP-CTFDepositorInsertAPP-CTF (APP Human)
UseTagsExpressionYeastMutationC-terminal fragment (aa 671-770)PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only