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Showing: 8601 - 8650 of 167150 results

  1. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid
    #197595
    Purpose
    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)
    Depositor
    Patrik Verstreken
    Insert
    mRuby2-T2A-GCaMP6f
    Use
    Lentiviral
    Promoter
    hSynI
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197595-plasmid-map-sequence-id-389365

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  2. pcDNA3-TRAM-CFP

    Plasmid
    #13027
    Depositor
    Doug Golenbock
    Insert
    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13027-plasmid-map-sequence-id-7425

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  3. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Expression
    Bacterial
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  4. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  5. pRSFDuet-galK

    Plasmid
    #193727
    Purpose
    Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK
    Depositor
    Martin Buck
    Insert
    galK (galK E. coli)
    Expression
    Bacterial
    Promoter
    J23113
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193727-plasmid-map-sequence-id-381922

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  6. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  8. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54537-plasmid-map-sequence-id-127234

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  9. mCherry-MyosinIIB-N-18

    Plasmid
    #55107
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. Pet28a_Human_H2B.1

    Plasmid
    #42630
    Depositor
    Joe Landry
    Insert
    Human H2B.1 (H2BC8 Human)
    Tags
    Untagged
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42630-plasmid-map-sequence-id-57745

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  12. AAV-syn-axon-jGCaMP8s-WPRE

    Plasmid
    #186043
    Purpose
    jGCaMP8s expression in axons under the control of the synapsin promoter
    Depositor
    Loren Looger
    Insert
    axon-jGCaMP8s
    Use
    AAV
    Tags
    GAP43-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186043-plasmid-map-sequence-id-370174

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  13. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid
    #186041
    Purpose
    jGCaMP8f expression in axons under the control of the synapsin promoter
    Depositor
    Loren Looger
    Insert
    axon-jGCaMP8f
    Use
    AAV
    Tags
    GAP43-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186041-plasmid-map-sequence-id-370171

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  14. rsGFP1-pBAD

    Plasmid
    #54851
    Purpose
    Localization: Protein Expression Vector , Excitation: 493, Emission: 510. Also called rsEGFP1
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and rsGFP1
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. SARS_pNIC-CTB10H

    Plasmid
    #153056
    Purpose
    Expression construct for producing Avi tagged Seryl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    SARS (SARS1 Human)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153056-plasmid-map-sequence-id-304476

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  16. Lenti sgRNA NGFR GFP out of frame

    Plasmid
    #155282
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280
    Depositor
    Martin Carroll
    Insert
    GFP out of frame IRES NGFR
    Available Since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155282-plasmid-map-sequence-id-310437

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  17. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Available Since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  19. pWALIUM10-GW-TurboID-V5

    Plasmid
    #196283
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196283-plasmid-map-sequence-id-385988

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  20. pLIX403-ccdB-Blast

    Plasmid
    #158560
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158560-plasmid-map-sequence-id-309876

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  21. pWALIUM10-V5-TurboID-GW

    Plasmid
    #196285
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196285-plasmid-map-sequence-id-384638

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  23. wt dynamin 2 pEGFP

    Plasmid
    #34686
    Depositor
    Sandra Schmid
    Insert
    dynamin 2 (Dnm2 Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    D406G, V664A, H692R and Q758R
    Promoter
    CMV
    Available Since
    Feb. 16, 2012
    Availability
    Academic Institutions and Nonprofits only
    34686-plasmid-map-sequence-id-142359

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  25. p3E_he1a:mCherry

    Plasmid
    #113881
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    mCherry
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113881-plasmid-map-sequence-id-223633

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  26. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163417-plasmid-map-sequence-id-321553

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  28. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  33. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  34. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Expression
    Mammalian
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

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  36. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  37. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

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  38. MIGR-mFoxP3

    Plasmid
    #24067
    Depositor
    Dan Littman
    Insert
    Foxp3 (Foxp3 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24067-plasmid-map-sequence-id-473505

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  39. pGR481

    Plasmid
    #213783
    Purpose
    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations
    Depositor
    Chang Liu
    Insert
    ura3*
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213783-plasmid-map-sequence-id-424628

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  40. mFGF5

    Plasmid
    #22087
    Depositor
    Gail Martin
    Insert
    FGF5 (Fgf5 Mouse)
    Use
    Bacterial
    Mutation
    V134G
    Available Since
    Oct. 1, 2009
    Availability
    Academic Institutions and Nonprofits only

  41. pIC162

    Plasmid
    #63070
    Purpose
    Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    Nuf2 (NUF2 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pSBtet-Bla/GFP

    Plasmid
    #198221
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198221-plasmid-map-sequence-id-389395

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  44. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  45. PG1424PGA

    Plasmid
    #74653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PG1424PGA
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  46. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

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  47. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  48. pAd5Rep2entire-ACGf

    Plasmid
    #237877
    Purpose
    AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.
    Depositor
    Ascend Advanced Therapies, Florian Sonntag
    Insert
    rep2 and E2A, E4, L4-22K/33K, VA RNA
    Use
    AAV
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237877-plasmid-map-sequence-id-475951

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  49. EBFP2-VASP-5

    Plasmid
    #55251
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    VASP (Vasp Mouse)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55251-plasmid-map-sequence-id-212996

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  50. pLVX-H3.3K27M-FLAG-BFP

    Plasmid
    #209434
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Expression
    Mammalian
    Mutation
    K27M
    Available Since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209434-plasmid-map-sequence-id-424833

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Showing: 8601 - 8650 of 167150 results