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pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dH6.2-2XG4S-actin

Plasmid

#73263

Purpose

Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)

Depositor

Marcel Bruchez

Article

Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

Insert

KozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)

Tags

The FAP and actin are fused with 2 copies of a G4…

Expression

Mammalian

Mutation

The dH6.2 FAP has one cysteine in each domain cha…

Promoter

CMV

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK8_WT

Plasmid

#82229

Purpose

Gateway Donor vector containing MAPK8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK8 (MAPK8 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM196A_WT

Plasmid

#81882

Purpose

Gateway Donor vector containing FAM196A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM196A (FAM196A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-NGL-3/LRRC4B [N51/6.2R]

Plasmid

#114493

Purpose

Mammalian Expression Plasmid of anti-NGL-3/LRRC4B (Rat). Derived from hybridoma N51/6.2.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody (Lrrc4b Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_MYC_p.E366Q

Plasmid

#82819

Purpose

Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MYC (MYC Human)

Use

Gateway entry vector

Mutation

L1M; E366Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 k46I

Plasmid

#27624

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMIR-REPORT-MYC CDS_WT

Plasmid

#108924

Purpose

MYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vector

Depositor

Jianjun Chen

Article

Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

Insert

MYC (MYC Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_BECN1_WT

Plasmid

#82100

Purpose

Gateway Donor vector containing BECN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BECN1 (BECN1 Human)

Use

Gateway entry vector

Mutation

A103V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
TMEM230-WT-IRES(isoform 1)

Plasmid

#99558

Purpose

mammalian expression of TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_G198R

Plasmid

#242465

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_L199R

Plasmid

#242466

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_L199G

Plasmid

#242467

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199G mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4

Plasmid

#242468

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The ANK3-4 mutations disrupt XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

ANK3-4 = R87A - E95A - K96A - W124G - K128G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-Tag100-hASB8_G198R

Plasmid

#242461

Purpose

Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

Tag100

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Fyn-SH2-AviTag

Plasmid

#214202

Purpose

Bacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

FYN kinase (FYN Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1022

Plasmid

#242503

Purpose

Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

ATG14 (ATG14 Human)
BECN1 (BECN1 Human)

Tags

ZZ-3xTEV

Expression

Mammalian

Promoter

CAG and CMV

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166D

Plasmid

#226720

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166D

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166D

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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