We narrowed to 9,742 results for: Tec

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NLS-dCas9-HA-NLS--GFP-p300

Plasmid

#183925

Purpose

p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-p300

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-Padron2

Plasmid

#172355

Purpose

Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

Depositor

Stefan Jakobs

Article

Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

Insert

Padron2

Tags

6xHisTag (N terminal on backbone)

Expression

Bacterial

Promoter

araBAD

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-EFGP

Plasmid

#176896

Purpose

mouse ILK cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-3xrGBD

Plasmid

#176108

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-Nono-sh5

Plasmid

#127651

Purpose

Knock-down of human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO shRNA (NONO Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST- IP2.0(codon for C.elegans)

Plasmid

#109216

Purpose

IP2.0 for C. elegans (Codon optimization and introns).

Depositor

Takeshi Ishihara

Article

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Tags

His-tag

Expression

Worm

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla 3A

Plasmid

#84872

Purpose

Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Tags

His

Expression

Bacterial

Mutation

3 C-terminal leucines have been replaced by alani…

Promoter

T7

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_Olig1

Plasmid

#64841

Purpose

A lentiviral vector for the expression of mouse Olig1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig1 (Olig1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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mPlum-Gamma-Tubulin-17

Plasmid

#55971

Purpose

Localization: Centrosomes, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
mWasabi-Mito-7

Plasmid

#56508

Purpose

Localization: Mitochondria, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mWasabi

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-dnMCAK

Plasmid

#208650

Purpose

Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

Depositor

Samuel Bakhoum

Article

Li et al Nature. 2023 Aug;620(7976):1080-1088. do

Insert

Kif2c (Kif2c Cricetulus griseus (Chinese hamster))

Tags

eGFP

Expression

Mammalian

Mutation

S92A , S106A , S108A, S112A , S186A, H530A, R534A…

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-ADARcd-YTHD422N

Plasmid

#194402

Purpose

ADAR1 catalytic domain E488Q fused to YTHD422N

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

ADARcd fused to YTHD422N (ADAR Human)

Tags

ADARcd-YTHD422N-HA

Expression

Mammalian

Mutation

D422N mutation in the YTH domain to enhance m6A b…

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

Plasmid

#113686

Purpose

An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

KRAB

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Expression

Mammalian

Mutation

D93A/D129A

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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