We narrowed to 18,889 results for: Tec

1. pBabe-puro-miR-10b sponge

   Plasmid

   #25043

   Depositor

   Bob Weinberg

   Article

   Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

   Insert

   miR-10b sponge
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

2. GGE142

   Plasmid

   #120735

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   Marker_Suc2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRFLIP42

   Plasmid

   #65518

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-Cit-attR1 and attR2-mCer-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRFLIP35

   Plasmid

   #65513

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-AFPt9-attR1 and attR2-TFPt9-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRFLIP32

   Plasmid

   #65511

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6578

   Plasmid

   #106130

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA NuiHA-loxP-nat-CUP1p

   Plasmid

   #131170

   Purpose

   PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Tags

   Nui-HA

   Expression

   Yeast

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos2-Talin-H-18

   Plasmid

   #57422

   Purpose

   Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin H
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Talin Head Domain

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pTn7·xylS

    Plasmid

    #122591

    Purpose

    Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLIPE-600n

    Plasmid

    #13541

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB602-mCherry-sfGFP

    Plasmid

    #213860

    Purpose

    For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    mCherry-sfGFP
    

    Expression

    Bacterial and Plant

    Promoter

    CaMV 35S

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-neoCV

    Plasmid

    #73889

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    neoCV
    

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBM19-sd4U

    Plasmid

    #179692

    Purpose

    Bacterial expression of base editor

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd4U] UGI-TALE4-DddA-G1397-C (T1413I)
    

    Expression

    Bacterial

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR-P5P4

    Plasmid

    #141064

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Septin 11 isoform III

    Plasmid

    #120319

    Purpose

    Septin 11 isoform III mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Expression

    Mammalian

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS414 GPD-Sis1

    Plasmid

    #18687

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Sis1 (SIS1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCY 3010-02

    Plasmid

    #36210

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yeast-enhanced (yE) polylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-hphMX4

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS406-PHO5-GFP-2xOsh2 PH domain [short]

    Plasmid

    #58829

    Purpose

    Expresses GFP-Tagged Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2xOsh2 PH domain [short] (OSH2 Budding Yeast)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only