We narrowed to 5,822 results for: pCas

1. pBECKP-spe

   Plasmid

   #117236

   Purpose

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEL063

   Plasmid

   #124895

   Purpose

   For genome editing in plant by SpCas9 variant(SpCas9-NG)

   Depositor

   Yong Zhang

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-Cetn1/1

   Plasmid

   #50718

   Purpose

   pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Inserts

   Cetn1 sgRNA1
   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh and hU6

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP416

   Plasmid

   #134996

   Purpose

   dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
   

   Use

   Synthetic Biology

   Tags

   3xFLAG, E coli Dam(N132A), and dCas9

   Expression

   Mammalian

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SiC-V1

   Plasmid

   #133041

   Purpose

   SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   dTomato

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-Hsp90 C(626-732)

   Plasmid

   #22483

   Depositor

   William Sessa

   Article

   Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

   Insert

   Hsp90alpha C domain (HSP90AA2P Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A_D10A-1x5

   Plasmid

   #58775

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. CANE-LV envelope

   Plasmid

   #86666

   Purpose

   pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

   Depositor

   Fan Wang

   Article

   Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

   Insert

   CANE-LV envelope
   

   Use

   Lentiviral, Mouse Targeting, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

9. p2T-CMV-HNHx-ABE8eHD-BlastR

   Plasmid

   #176478

   Purpose

   A-to-G base editor with alternative targeting scope

   Depositor

   Gerald Schwank

   Article

   Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

   Insert

   HNHx-ABE8eHD
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_085

    Plasmid

    #158583

    Purpose

    Lentiviral expression of SpCas9; P2A site provides mKate2

    Depositor

    John Doench, David Root

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    SpCas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaV2.1 (pCR3.1)

    Plasmid

    #26573

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pFB-Flag-MyoD-E12

    Plasmid

    #25990

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. sABE v3.22 Lenti_1

    Plasmid

    #209555

    Purpose

    pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT-9218

    Plasmid

    #124225

    Purpose

    Bacterial SpCas9 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK0_003

    Plasmid

    #123925

    Purpose

    Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Cas9-sgRNA destination
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AYBEv0.1

    Plasmid

    #193966

    Purpose

    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    

    Expression

    Mammalian

    Promoter

    hU6, EF1a, CBh

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG184

    Plasmid

    #165604

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    'CAGCG' PAM replacing the 'CGGCG&#…

    Promoter

    lac

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CMV-HNHx-ABEmax7.10HD-BlastR

    Plasmid

    #176477

    Purpose

    A-to-G base editor with alternative targeting scope

    Depositor

    Gerald Schwank

    Article

    Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

    Insert

    HNHx-ABEmax7.10HD
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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