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pc3XB-ZF75

Plasmid

#13209

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF75

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF76

Plasmid

#13210

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF76

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF78

Plasmid

#13212

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF78

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF80

Plasmid

#13214

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF80

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF82

Plasmid

#13216

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF82

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF85

Plasmid

#13219

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF85

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF86

Plasmid

#13220

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF86

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF87

Plasmid

#13221

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF87

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF90

Plasmid

#13224

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF90

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF91

Plasmid

#13225

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF91

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF94

Plasmid

#13228

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF94

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF97

Plasmid

#13231

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF97

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF99

Plasmid

#13233

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF99

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF101

Plasmid

#13235

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF101

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF104

Plasmid

#13238

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF104

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF105

Plasmid

#13239

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF105

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p077 pCS xTRPS1 (M)

Plasmid

#11047

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

Two Cys residues in the GATA-type zinc finger wer…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG BacMam C term 3C eGFP His8

Plasmid

#160687

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP His8

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-N-RAM-d2tTA-TRE-mKate2

Plasmid

#140275

Purpose

N-RAM, the Npas4-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Has Service

AAV1

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

PAH1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PAmCherry1-N1-Tubbyc

Plasmid

#202677

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505): PAmCherry1 (Tub Mouse)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SigmaR1-mNeonGreen

Plasmid

#226566

Purpose

Encodes SigmaR1 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-MAPPER

Plasmid

#117721

Purpose

MAPPER Selectively Labels ER-PM Junctions

Depositor

Jen Liou

Article

Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

Insert

MAPPER

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-HA.Tetherin

Plasmid

#41069

Depositor

Paul Spearman

Article

Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

Insert

Tetherin (BST2 Human)

Use

Retroviral

Tags

HA and IRES Puro

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWM_12x601_25bpLinker

Plasmid

#157788

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_25bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Human ICAM-1

Plasmid

#8632

Depositor

Timothy Springer

Article

Staunton et al Cell 1988 Mar 25;52(6):925-33.

Insert

ICAM-1 (ICAM1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mStayGold-EFS-Puro

Plasmid

#227256

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,506 results for: Emb