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We narrowed to 14,660 results for: flag

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Showing: 6921 - 6960 of 14660 results

  3. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  8. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  9. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  10. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

    Plasmid
    #139444
    Purpose
    Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    sequence codon-optimized
    Promoter
    SFFV
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139444-plasmid-map-sequence-id-308466

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  12. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  16. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  19. pKlab897-HBEGF-2a-3xFLAG-BRCA1(full-length)-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248988
    Purpose
    This plasmid expresses the BRCA1 protein sequence, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248988-plasmid-map-sequence-id-495729

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  22. pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

    Plasmid
    #248034
    Purpose
    Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells
    Depositor
    Joshua Leonard
    Article
    Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.
    Insert
    3xFLAG-tagged Neoleukin-2/15
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    human EF1a
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248034-plasmid-map-sequence-id-497728

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  24. pANC56(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-KKYL-IRES-EGFP)

    Plasmid
    #248355
    Purpose
    Lentiviral vector that can express hAqp1_ER export based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker
    Depositor
    Arnab Mukherjee
    Article
    Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi
    Insert
    FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-KKYL
    Use
    Lentiviral
    Tags
    3x TEV site, KKYL and FLAG (internal in Aqp1)
    Promoter
    EF-1a
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248355-plasmid-map-sequence-id-497465

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  26. pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP)

    Plasmid
    #248354
    Purpose
    Lentiviral vector that can express hAqp1_degron based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker
    Depositor
    Arnab Mukherjee
    Article
    Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi
    Insert
    FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron
    Use
    Lentiviral
    Tags
    3x TEV site, FKBP12 degron and FLAG (internal in …
    Promoter
    EF-1a
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    248354-plasmid-map-sequence-id-493085

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  28. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

    Plasmid
    #227158
    Purpose
    For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    cyp-33e1 (cyp-33E1 Nematode)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 295 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227158-plasmid-map-sequence-id-458998

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  29. pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

    Plasmid
    #227164
    Purpose
    For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 14, cysteine 82, and cysteine 43…
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227164-plasmid-map-sequence-id-459002

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  30. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

    Plasmid
    #240514
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-20 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240514-plasmid-map-sequence-id-477194

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  31. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid
    #240515
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-125a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240515-plasmid-map-sequence-id-481869

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  32. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid
    #240516
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-151a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240516-plasmid-map-sequence-id-477188

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  33. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid
    #240517
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-221 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240517-plasmid-map-sequence-id-477189

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  34. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

    Plasmid
    #226115
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-107 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226115-plasmid-map-sequence-id-448321

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  35. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid
    #226109
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-92a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226109-plasmid-map-sequence-id-448313

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  36. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

    Plasmid
    #226110
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-378a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226110-plasmid-map-sequence-id-448316

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  37. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

    Plasmid
    #226112
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-33b Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226112-plasmid-map-sequence-id-448314

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Showing: 6921 - 6960 of 14660 results