We narrowed to 18,440 results for: Met

1. pEGFP-Map7-fulllength

   Plasmid

   #46076

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-fulllength (Map7 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW149

   Plasmid

   #122248

   Purpose

   pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

   Depositor

   Sarah Walker

   Article

   Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

   Insert

   TIF4631 (TIF4631 Budding Yeast)
   

   Tags

   Intein-Chitin-binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNF146-c005

   Plasmid

   #173091

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   RNF146 (RNF146 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   R99-L183, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Expression

   Yeast

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS-mCardinal2

   Plasmid

   #52631

   Purpose

   bacterial expression vector with far-red FP mCardinal2

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal2
   

   Expression

   Bacterial

   Available Since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS275

   Plasmid

   #34972

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Mutation

   T406A, T407A, I532A

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

   Plasmid

   #185802

   Purpose

   one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
   

   Expression

   Mammalian

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. anti-Notch2_B6-pBIOCAM5

    Plasmid

    #39347

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
    

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. GDAP2-c005

    Plasmid

    #173110

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GDAP2 (GDAP2 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    M1-N231

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Promoter

    Ptet

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Burden Monitor phi80 version

    Plasmid

    #66074

    Purpose

    The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Yellow Cameleon-Nano140/pcDNA3

    Plasmid

    #51966

    Purpose

    mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano140
    

    Expression

    Mammalian

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121185

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Tet-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Mutation

    OsTIR1 codon optimized for human cells

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MBD-IFP2.0C

    Plasmid

    #215748

    Purpose

    Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MBD_R44Q-IFP2.0C

    Plasmid

    #215749

    Purpose

    Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xHP1bCD-IFP2.0C

    Plasmid

    #215750

    Purpose

    Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD-IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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