We narrowed to 11,662 results for: cel.2

1. myc-actin-NES-1

   Plasmid

   #60618

   Purpose

   Encodes MYC tagged beta-actin with a mutation in the NES

   Depositor

   Primal de Lanerolle

   Article

   Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   MYC

   Expression

   Bacterial and Mammalian

   Mutation

   I178A, L180A

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. GST-RAPH1-F2

   Plasmid

   #194864

   Purpose

   Express the RAPH1 fragment (535-868) tagged to GST in bacteria.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 1062-1250 (RAPH1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1062-1250

   Promoter

   Tac

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_FGF21

   Plasmid

   #182406

   Purpose

   Bacterial expression of fgf21

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   fgf21 (FGF21 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

   Plasmid

   #182393

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ
   CydAB-mNeongreen
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

   Plasmid

   #192373

   Purpose

   To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-TB-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

   Plasmid

   #192372

   Purpose

   To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

   Plasmid

   #192377

   Purpose

   To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

   Plasmid

   #192378

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-U6a-U6c-mfn2 guide

   Plasmid

   #121993

   Purpose

   An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mfn2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-U6a-U6c-opa1 guide

    Plasmid

    #121995

    Purpose

    An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    opa1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9

    Plasmid

    #100162

    Purpose

    Expresses ZFYV9

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    ZFYV9 (ZFYVE9 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-Sema4b-ISH-2kb

    Plasmid

    #68030

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4B (Sema4b Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRII-Sema4f-ISH-2kb

    Plasmid

    #68033

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4F (Sema4f Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hs-FL-Kek1-myc2

    Plasmid

    #41170

    Depositor

    Norbert Perrimon

    Article

    Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

    Insert

    Kek1 (kek1 Fly)
    

    Use

    Tags

    2X myc

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

15. XE156 delta LZ-XDpr-CS2+

    Plasmid

    #16766

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    delta LZ-XDpr (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    deletion of first 129 amino acids, including the …

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

16. XE154 XDpr-pt mutant-CS2+

    Plasmid

    #16768

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    XDpr-pt mutant (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    last four amino acids mutated to MNTV instead of …

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

    Plasmid

    #159130

    Purpose

    expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

    Plasmid

    #159128

    Purpose

    expresses KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST40-2XFL-Src-S4-UD-mCherry

    Plasmid

    #140306

    Purpose

    Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X FL

    Expression

    Mammalian

    Mutation

    contains only the SH4 and UD regions fused to mCh…

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST40-2XFL-Src-S4-UD-S32

    Plasmid

    #140303

    Purpose

    Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-Foxo1-ADA 6KR

    Plasmid

    #17561

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1-ADA 6KR (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-Foxo1-ADA 6KQ

    Plasmid

    #17563

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA 6KQ (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

    Plasmid

    #159127

    Purpose

    expresses FBXO22 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    FBXO22 (FBXO22 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCW57.1 _Gata6

    Plasmid

    #73537

    Purpose

    Inducible lentiviral expression of Gata6

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST40-2XFL-Src-S4-S32-KD

    Plasmid

    #140299

    Purpose

    Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-myc3-Mdm2 D367A

    Plasmid

    #52058

    Purpose

    expression of non-cleavable Mdm2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Mdm2 D367A (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    D367A

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pMYs-MFN2-IRES-mitoRFP

    Plasmid

    #121997

    Purpose

    Expression of MFN2 and mitoRFP in mammalial cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    MFN2-IRES-mitoRFP (MFN2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

    Plasmid

    #159132

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11F

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge