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We narrowed to 6,763 results for: poly

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Showing: 5221 - 5250 of 6763 results

  2. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  6. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  10. pLEW13

    Plasmid
    #24007
    Depositor
    George Cross
    Insert
    T7 RNA Polymerase
    Use
    Trypanosome expression
    Available Since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24007-plasmid-map-sequence-id-11278

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  11. pAcGFP-IVT

    Plasmid
    #205145
    Purpose
    IVT template of AcGFP with MCS in frame and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205145-plasmid-map-sequence-id-404881

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  12. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

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  13. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205146-plasmid-map-sequence-id-404879

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  15. SV40-MCS-Z1

    Plasmid
    #127639
    Purpose
    Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance
    Depositor
    Jordan Green
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    SV40 promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127639-plasmid-map-sequence-id-273360

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  16. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-495367

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  17. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205148-plasmid-map-sequence-id-404883

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  18. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165566-plasmid-map-sequence-id-340094

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  19. pAcRP67A prM/E YFV

    Plasmid
    #52883
    Purpose
    expression of Yellow Fever Virus VLPs in baculovirus
    Depositor
    Yorgo Modis
    Insert
    prM/E
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. Hsp68-CreER(TM) (DM#264)

    Plasmid
    #15028
    Depositor
    Douglas Melton
    Insert
    Cre-ER(TM)
    Use
    Cre/Lox; For making transgenic constructs
    Tags
    Hsp68 promoter and polyA
    Expression
    Mammalian
    Available Since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    15028-plasmid-map-sequence-id-152167

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  21. pAB-LIC C

    Plasmid
    #62288
    Purpose
    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    8XHis tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pBS mTSP4

    Plasmid
    #12415
    Depositor
    Paul Bornstein
    Insert
    TSP4 (Tsp4 Mouse)
    Expression
    Bacterial
    Mutation
    2 kb fragment from third type 2 repeat to polyA t…
    Available Since
    Oct. 23, 2006
    Availability
    Academic Institutions and Nonprofits only

  23. pEGGsH6_MCS_SHORT_outFrame

    Plasmid
    #211439
    Purpose
    Vector backbone for random polymerase chain reaction screen
    Depositor
    Helmut Pospiech
    Insert
    MCS-tev cleavage site-EGFP-6xHis-Tag
    Expression
    Bacterial
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    211439-plasmid-map-sequence-id-421993

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  24. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  25. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst HF
    Use
    Synthetic Biology
    Available Since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

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  26. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  27. pCR175

    Plasmid
    #240640
    Purpose
    Template for in vitro transcription of mTurquoise2 mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mTurquoise2
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240640-plasmid-map-sequence-id-483016

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  28. pCR178

    Plasmid
    #240641
    Purpose
    Template for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mScarlet-I
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240641-plasmid-map-sequence-id-482342

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  29. pEGGsH6-MCS_SHORT_inFrame

    Plasmid
    #211438
    Purpose
    Vector backbone for random polymerase chain reaction screen
    Depositor
    Helmut Pospiech
    Insert
    MCS-tev cleavage site-EGFP-6xHis-Tag
    Expression
    Bacterial
    Available Since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    211438-plasmid-map-sequence-id-420494

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  30. pTYB3-T4gp43(WT)

    Plasmid
    #228203
    Purpose
    Expression in BL21(DE3) of WT T4 DNA polymerase
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    228203-plasmid-map-sequence-id-472938

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Showing: 5221 - 5250 of 6763 results