We narrowed to 4,337 results for: U6 gRNA

1. HEK3_pegF_44bp

   Plasmid

   #174854

   Purpose

   Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegF_44bp insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HEK3_pegR_44bp

   Plasmid

   #174855

   Purpose

   Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegR_44bp insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBC2103-reci

   Plasmid

   #117172

   Purpose

   U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_arCas9 without NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBC2101-reci

   Plasmid

   #117170

   Purpose

   U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_wtCas9 with SV40 NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-crRNA-MS2

   Plasmid

   #207624

   Purpose

   Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   crRNA-MS2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZR060_CROPseq-sgCtrl-CRISPRi

   Plasmid

   #180269

   Purpose

   Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   No-Target Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR069_CROPseq-sgPTPRC-CRISPRi

   Plasmid

   #180271

   Purpose

   Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   PTPRC (CD45) sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZR064_CROPseq-sgCD4-CRISPRi

   Plasmid

   #180270

   Purpose

   Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   CD4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDAS12342_PEAR-GFP_2in1

    Plasmid

    #177184

    Purpose

    PEAR-GFP plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-TP53-1

    Plasmid

    #121917

    Purpose

    Encodes sgRNA targeting exon 4 of TP53

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-TP53-2

    Plasmid

    #121918

    Purpose

    Encodes sgRNA targeting exon 9 of TP53

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cdk13_intron3_sg4_pX458

    Plasmid

    #127339

    Purpose

    sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cdk13_intron4_sg3_pX330

    Plasmid

    #127340

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIL-eGFP-shACTN1

    Plasmid

    #52676

    Purpose

    shRNA against α-actinin-1 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN1 (Actn1 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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