We narrowed to 10,981 results for: Vars;

1. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

   Plasmid

   #124621

   Purpose

   Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   ADAR2(E488Q)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutant

   Promoter

   CMV

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Cre stuffer v3

   Plasmid

   #158030

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-GFP-Flag-UTR

   Plasmid

   #183235

   Purpose

   To express FLAG tagged eGFP

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   EGFP
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

   Plasmid

   #177183

   Purpose

   plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   sgRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Vimentin-C-18

   Plasmid

   #55157

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-SlugCBE-NNG-P2A-puro

   Plasmid

   #214355

   Purpose

   Expresses SlugCBE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCBE-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK-tetO2

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nanog-2A-GFP

    Plasmid

    #59994

    Purpose

    Inserts GFP immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nanog-2A-mCherry

    Plasmid

    #59995

    Purpose

    Inserts mCherry immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTFP1-Vinculin-23

    Plasmid

    #55516

    Purpose

    Localization: Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ECFP-ER-0

    Plasmid

    #55342

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    ER (CALR Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP HDAC5 WT

    Plasmid

    #32211

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pKP3915

    Plasmid

    #113335

    Purpose

    Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Insert

    Artificial Transcription Factor (McIsaac et al, 2013)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-NG-PE2-BSD

    Plasmid

    #176933

    Purpose

    Expresses PE2-NG variant in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Insert

    PE2-NG variant
    

    Use

    Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJE53

    Plasmid

    #194153

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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