We narrowed to 62,126 results for: cloning

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pCMU-PMr

Plasmid

#61180

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-IFP2.0 T2A HO1

Plasmid

#59427

Purpose

Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

Depositor

Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Insert

IFP2.0T2AHO1 IRES EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Human, Mouse)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAC149-pCR8-dCas9VP160

Plasmid

#48221

Purpose

dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR and Gateway Cloning

Tags

HA Tag and VP160

Mutation

D10A;H840A nuclease-deficient

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAC91-pmax-dCas9VP64

Plasmid

#48223

Purpose

dCas9VP64 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR

Tags

HA Tag and VP64

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSAM

Plasmid

#45174

Depositor

Jumi Shin

Article

Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

Type

Empty backbone

Tags

6xHis and 6xHis/Thrombin/T7

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
gRNA_DNMT3a-T1

Plasmid

#41821

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T1 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti6-BMPR1A(Q233D)-V5

Plasmid

#35638

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

BMP Receptor Type1A Q233D (BMPR1A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glutamine 233 to Aspartate

Promoter

CMV

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-MYT1L

Plasmid

#32926

Purpose

Retroviral expression of human Myt1L

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

MYT1L (MYT1L Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Ngn2

Plasmid

#32928

Purpose

Retroviral expression of mouse Ngn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
phMYT1L-N106

Plasmid

#31782

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

myelin transcription factor 1-like (MYT1L Human)

Use

Lentiviral

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 (Ulk2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
flk1 promoter

Plasmid

#26436

Depositor

Didier Stainier

Article

Jin et al Development. 2005 Dec . 132(23):5199-209

Insert

flk1 promoter

Use

Topo cloning vector

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST_TAF15

Plasmid

#26379

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LATS1

Plasmid

#23665

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LATS1 (LATS1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MARK3

Plasmid

#23716

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MARK3 (MARK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RIPK1

Plasmid

#23442

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK1 (RIPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway Cloning

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
PB-TET-mSox2

Plasmid

#20908

Purpose

piggyBac vector with tet inducible mSox2

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Sox2 (Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPpA-ACN

Plasmid

#20343

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

EGFPpA-ACN

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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