We narrowed to 9,643 results for: SUB

1. pBluescript-Evf1

   Plasmid

   #99479

   Purpose

   Subcloning of the Evf1 long non-coding RNA

   Depositor

   Jhumku Kohtz

   Article

   Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

   Insert

   Evf1
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. KW1

   Plasmid

   #21211

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek2 (MAP2K2 Human)
   

   Use

   Subclone vector

   Mutation

   KW71, constitutive active

   Available Since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pCZGY2845

   Plasmid

   #87763

   Purpose

   Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pnmr-1-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Pnmr-1

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. W1

   Plasmid

   #21208

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (MAP2K1 Human)
   

   Use

   Subclone vector

   Available Since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pWS1037

   Plasmid

   #123049

   Purpose

   G_ subunit

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Gpa1 [3-4a]
   

   Expression

   Yeast

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-μ2-(HA)3

   Plasmid

   #198178

   Purpose

   Expression of HA-tagged AP-2 μ2 in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

   Insert

   AP-2 μ2
   

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent substitution in codon 80

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1hisA cdc25c S216A

   Plasmid

   #10968

   Depositor

   Toren Finkel

   Article

   Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

   Insert

   cdc25c S216A (CDC25C Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S216A. Serine to Alanine substitution at aa216.

   Available Since

   Nov. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

8. GST cdc25c C330S C377S

   Plasmid

   #10970

   Depositor

   Toren Finkel

   Article

   Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

   Insert

   cdc25c C330S C377S (CDC25C Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C330S C377S. Cysteine to Serine substitution at …

   Available Since

   Nov. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

9. p40 FL

   Plasmid

   #19013

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. wt Cus2p

    Plasmid

    #73046

    Purpose

    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Promoter

    T7

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1hisA cdc25c C330S

    Plasmid

    #10966

    Depositor

    Toren Finkel

    Article

    Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

    Insert

    cdc25c C330S (CDC25C Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    C330S. Cysteine to Serine substitution at aa330.

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pTYB1-T4gp46

    Plasmid

    #228211

    Purpose

    Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    46
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. NFfshb-t2a-GFP

    Plasmid

    #194356

    Purpose

    over expression vector for killifish fshb tagged with GFP

    Depositor

    Itamar Harel

    Article

    Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

    Insert

    follicle stimulating hormone beta subunit
    

    Use

    Lentiviral

    Tags

    t2a-GFP

    Expression

    Mammalian

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNC-RFP-N

    Plasmid

    #193412

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNC-RFP-C

    Plasmid

    #193413

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNC-mCherry-N

    Plasmid

    #193414

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNC-mCherry-C

    Plasmid

    #193415

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNC-CFP-N

    Plasmid

    #193410

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNC-CFP-C

    Plasmid

    #193411

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNC-Amp-GFP-C

    Plasmid

    #193417

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNC-Amp-GFP-N

    Plasmid

    #193416

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2+-dCas9-olEzh2(deltaSET)

    Plasmid

    #180217

    Purpose

    Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

    Depositor

    Hiroyuki Takeda

    Article

    Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

    Insert

    enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)
    

    Tags

    FLAG and dCas9

    Expression

    Bacterial

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pQLinkHD_LSSmOrange

    Plasmid

    #118860

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    LSSmOrange
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    A44V,F84L, W145M, I165D, M167L, G202D substituti…

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. MPS-1-cmyc

    Plasmid

    #113059

    Purpose

    Expresses worm Myc-tagged MPS-1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

    Insert

    MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. KHT-1

    Plasmid

    #113054

    Purpose

    Expresses worm KHT-1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al EMBO J. 2009 Jun 3;28(11):1601-11. doi:

    Insert

    K+ channel for Habituation to Tap subunit 1
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMVmyc-ORF73SOCS

    Plasmid

    #21546

    Depositor

    J Simas

    Article

    Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

    Insert

    ORF73 (ORF73 MuHV-4)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    amino acids at positions 199, 202, 203 and 206 we…

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLG015 (RADAR)

    Plasmid

    #157893

    Purpose

    Expresses the RADAR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RADAR subtype containing auxillary gene rdrD
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pLEXSY-hyg2.1_LtIFT43_Flag

    Plasmid

    #194433

    Purpose

    Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

    Depositor

    Alan Brown

    Article

    Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

    Insert

    Intraflagellar transport complex subunit 43
    

    Use

    Leishmania tarentolae expression

    Tags

    3X Flag and Linker

    Promoter

    L.donovani promoter elements

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. mCherry-Sec61β

    Plasmid

    #172445

    Purpose

    Expression of ER protein Sec61beta with an N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Protein transport protein Sec61 subunit beta
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-4mtD3cpv

    Plasmid

    #36324

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
    

    Tags

    First 29 aa of human COX subunit 8a

    Promoter

    CMV

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCZGY3163

    Plasmid

    #101248

    Purpose

    GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp11::rps-18 (rps-18 Nematode, Synthetic)
    

    Use

    Gateway

    Tags

    GFP11

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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36. p426MET25_sfpHluorin (MRV55)

    Plasmid

    #115697

    Purpose

    superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    sfpHluorin
    

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-p47PX(aa2-132)

    Plasmid

    #19007

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p47phox (NCF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa2-132 (PX domains)

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Expression

    Bacterial

    Promoter

    lac UV5

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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