We narrowed to 14,621 results for: RING
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Plasmid#205543PurposeExpress EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP-Myc
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB88
Plasmid#199321PurposeCombine with Gal4 to direct TeNL expressionDepositorInsert15xUAS::delta pes-10::::TeNL::let-858 3'UTR
ExpressionWormMutationKD for calcium is 250 nM, 1 synthetic intronPromoter15xUAS::delta pes-10Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB109
Plasmid#199322PurposeCombine with Gal4 to direct CaMBI 300 expressionDepositorInsert15xUAS::delta pes-10::::CaMBI::let-858 3'UTR
ExpressionWormMutationOrange CaMBI with 300 nM KD for calciumPromoter15xUAS:::delta pes-10Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT186
Plasmid#204513PurposeBacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertPAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
Tags6xHis, MBPExpressionBacterialPromoterT7lacAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT520
Plasmid#204519PurposeBacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
Tags6xHis, MBPExpressionBacterialPromoterT7lacAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT320
Plasmid#204517PurposeBacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertInternal fragment of him-1 (C. elegans) (him-1 Nematode)
Tags6xHis, MBPExpressionBacterialPromoterT7lacAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Silene_conica _GlnRS_MitoTP_pK7FWG2
Plasmid#202654PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _LysRS_MitoTP_pK7FWG2
Plasmid#202655PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Agrostemma_githago _LysRS_MitoTP_pK7FWG2
Plasmid#202656PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _TyrRS_MitoTP_pK7FWG2
Plasmid#202657PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
Plasmid#202658PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_MitoTP_pK7FWG2
Plasmid#202659PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_PlastidTP_pK7FWG2
Plasmid#202660PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2
Plasmid#202661PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria
TagsGreen Florescent ProtienExpressionPlantAvailable SinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-2T_RV-A89_VP1
Plasmid#202073PurposeBacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-A89 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP
Plasmid#188272PurposeMammalian expression, Genome editingDepositorInsertAaCas12b(Q119F/E475R/E758R)
UseCRISPRExpressionMammalianMutationnonePromoterCAGAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT011-ZF6 1x FLAG-synTF-PPF
Plasmid#202501PurposeExpress "PPF" synTF (ZF6)DepositorInsertp65-ZF6
Tags1x FLAG and Palmitoylation-Palmitoylation-Farnesy…ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVioCD53
Plasmid#191130PurposeExpression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoterDepositorInsertsUseSynthetic BiologyMutationC-terminal truncation from 264th amino acidPromoterJ23105 constitutive promoterAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
OsMTI-1B Oryza sativa Metallothionein 1B
Plasmid#200295PurposeBacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)
TagsChiting Binding Domanin-InteinExpressionBacterialAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-AmCyan-P2A-NptII-9
Plasmid#197742PurposePhytobrick (MoClo) Level 0 PartDepositorInsertAmCyan-P2A-NptII bicistronic CDS
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMX-Mef2c-puro
Plasmid#198228PurposeRetroviral expression of Mef2cDepositorInsertMef2c (Mef2c Mouse)
UseRetroviralAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL2114 pARF19 GG0-GG1
Plasmid#195909PurposeLevel 0 pARF19 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF19 (ARF19 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_FusionRed-SYFP2
Plasmid#189760PurposeDual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysisDepositorInsertFusionRed-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-E208Q_E
Plasmid#146196PurposeInsect Expression of DmMe31B-E208QDepositorInsertDmMe31B-E208Q (me31B Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-K108A_E
Plasmid#146197PurposeInsect Expression of DmMe31B-K108ADepositorInsertDmMe31B-K108A (me31B Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-mut1_E
Plasmid#146198PurposeInsect Expression of DmMe31B-mut1DepositorInsertDmMe31B-mut1 (me31B Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-E208Q_E
Plasmid#146201PurposeInsect Expression of DmMe31B-E208QDepositorInsertDmMe31B-E208Q (me31B Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-K108A_E
Plasmid#146202PurposeInsect Expression of DmMe31B-K108ADepositorInsertDmMe31B-K108A (me31B Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I16 tUBQ1 GG5-GG6
Plasmid#195898PurposeLevel 0 tUBQ1 terminator with GG5 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I15 tUBQ1 GG4-GG6
Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV R221C SPOP 28-359
Plasmid#194116PurposeR221C His SUMO SPOP expression plasmidDepositorAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-T7- YFP
Plasmid#186568Purposep15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
ExpressionMammalianAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N2
Plasmid#174001PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O2
Plasmid#174002PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265L2
Plasmid#174000PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2265
Plasmid#175962PurposeFor expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
Tags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ347-361, replace with 702-716PromoteraraBADAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEYFP(Q69K)
Plasmid#190746PurposeExpression of mEYFP(Q69K)-tagged CD86 in mammalian cells.DepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro
Plasmid#167899PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2
Plasmid#167897PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro
Plasmid#167903PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_dPC_2xChromo
Plasmid#179398PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromoDepositorInsertdPC_2xChromo
TagsAviTag and EGFPExpressionMammalianPromoterCAGGSAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only