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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
3xFKBP-myc

Plasmid

#20213

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-CDK4-shRNA

Plasmid

#25788

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK4 (CDK4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN (2-119)

Plasmid

#208867

Purpose

Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

aa 2-119 only

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MSP2279

Plasmid

#70706

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A and H557A in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD3_2

Plasmid

#36372

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD3 (MBD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pDF0044 CjcCas7-11 full locus with synthetic array

Plasmid

#172502

Purpose

Encodes the CjcCas7-11 full locus for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 full locus with synthetic array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1 (WT)

Plasmid

#208871

Purpose

For expression in E. coli of NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUASt-GBP-Trbo

Plasmid

#179996

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Tags

Trbo

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_2

Plasmid

#36332

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-Cyp26a1

Plasmid

#13457

Depositor

T. Michael Underhill

Article

Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

Insert

Cyp26a1 (Cyp26a1 Mouse)

Expression

Mammalian

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
Flag-Craf S338A

Plasmid

#118335

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf S338A (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S338A

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-FL-3'UTR

Plasmid

#113088

Purpose

Luciferase reporter containing PAK4- FL-3'UTR

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A)

Plasmid

#41586

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-UBC-9

Plasmid

#87055

Purpose

Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

UBC-9 (ubc-9 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_3

Plasmid

#36333

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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